Huang Ting, Planck Stephen R, Rosenbaum James T, Lee Ellen J
Key Laboratory of Ophthalmology of the Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China.
Ocul Immunol Inflamm. 2009 Jul-Aug;17(4):257-64. doi: 10.1080/09273940902802683.
In contrast to penetrating keratoplasty (PK), the donor cornea in lamellar keratoplasty (LK) remains separated from the host aqueous humor. There is debate about relative merits of each approach, but experimental comparisons have never been performed in animal models. Therefore, the authors developed a murine LK model.
For allogeneic PK and LK surgeries, corneas of C57BL/6 mice were transplanted to BALB/c mice, assessed by slit lamp, and scored for opacity, edema, and neovascularization up to 46 d post-transplantation. Additional PK or LK surgeries were performed for histological assessment.
Graft rejection rate was less in LK vs. PK (69.2 vs. 100%), as was neovascularization (84.6 vs. 100%). In LK, inflammatory cells infiltrated primarily the button; in PK, heavier infiltration was observed throughout the cornea.
This study demonstrates the feasibility of LK in mice and presents data suggesting that the inflammatory response in LK differs from that in PK.
与穿透性角膜移植术（PK）不同，板层角膜移植术（LK）中的供体角膜与宿主房水保持分离。关于每种方法的相对优点存在争议，但从未在动物模型中进行过实验比较。因此，作者开发了一种小鼠LK模型。
对于同种异体PK和LK手术，将C57BL/6小鼠的角膜移植到BALB/c小鼠上，通过裂隙灯评估，并在移植后长达46天对混浊、水肿和新生血管形成进行评分。进行额外的PK或LK手术以进行组织学评估。
LK组的移植物排斥率低于PK组（69.2%对100%），新生血管形成情况也是如此（84.6%对100%）。在LK中，炎性细胞主要浸润移植片；在PK中，整个角膜观察到更严重的浸润。
本研究证明了LK在小鼠中的可行性，并提供数据表明LK中的炎症反应与PK中的不同。
