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自组装脂质双层膜的结构与取向织构

Structure and orientational texture of self-organizing lipid bilayers.

作者信息

Watkins E B, Miller C E, Mulder D J, Kuhl T L, Majewski J

机构信息

Biophysics Graduate Group, University of California, Davis, CA 95616, USA.

出版信息

Phys Rev Lett. 2009 Jun 12;102(23):238101. doi: 10.1103/PhysRevLett.102.238101. Epub 2009 Jun 10.

DOI:10.1103/PhysRevLett.102.238101
PMID:19658974
Abstract

The structure of single supported dipalmitoyl-phosphatidylcholine bilayers prepared by vesicle fusion or Langmuir-Blodgett-Schaeffer (LBS) deposition techniques was characterized by x-ray reflectivity and grazing incidence diffraction in bulk water. LBS bilayers display symmetric leaflets similar to monolayer structures, while vesicle fusion yields more inhomogeneous bilayers. Diffraction establishes that lipids are always coupled across the bilayer even when leaflets are deposited independently and suggests the existence of orientational texture.

摘要

通过囊泡融合或朗缪尔-布洛杰特-谢弗(LBS)沉积技术制备的单支撑二棕榈酰磷脂酰胆碱双层膜的结构,在大量水中通过X射线反射率和掠入射衍射进行了表征。LBS双层膜显示出与单层结构相似的对称小叶,而囊泡融合产生的双层膜更不均匀。衍射表明,即使小叶是独立沉积的,脂质在整个双层膜中总是相互耦合的,并表明存在取向织构。

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