Division of Oral Pathology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
J Oral Pathol Med. 2010 Jan;39(1):87-93. doi: 10.1111/j.1600-0714.2009.00807.x. Epub 2009 Jul 31.
To investigate the roles of stem cell-related molecules in oncogenesis and cytodifferentiation of odontogenic tumors, expression of CD133, Bmi-1, and ATP-binding cassette subfamily G member 2 (ABCG2) was analyzed in ameloblastic tumors as well as in tooth germs.
Tissue specimens of 12 tooth germs, 47 ameloblastomas, and six malignant ameloblastic tumors were examined using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry to determine the expression of CD133, Bmi-1, and ABCG2.
mRNA expression of CD133, Bmi-1, and ABCG2 was detected in all samples of normal and neoplastic odontogenic tissues. Immunohistochemical reactivity for CD133 and Bmi-1 was evident in odontogenic epithelial cells neighboring the basement membrane in tooth germs, ameloblastomas, and metastasizing ameloblastomas, and ameloblastic carcinomas and clear cell odontogenic carcinomas showed reactivity for CD133 and Bmi-1 in most neoplastic cells. The level of CD133 immunoreactivity in malignant ameloblastic tumors was significantly higher than the levels in tooth germs and ameloblastomas. Immunoreactivity for ABCG2 in odontogenic epithelial components was detected in some ameloblastic tumors but not in tooth germ tissues. Some granular neoplastic cells in granular cell ameloblastomas showed ABCG2 expression. The level of ABCG2 immunoreactivity in malignant ameloblastic tumors was significantly higher than that in tooth germs.
Expression of CD133, Bmi-1, and ABCG2 in tooth germs and ameloblastic tumors suggests that stem cell-related molecules might control the maintenance of odontogenic tissues. Expression of these molecules is possibly involved in oncogenesis, cell differentiation, and malignant potential of odontogenic epithelium.
为了研究干细胞相关分子在牙源性肿瘤发生和细胞分化中的作用，本研究分析了牙源性肿瘤和牙胚中 CD133、Bmi-1 和三磷酸腺苷结合盒亚家族 G 成员 2（ABCG2）的表达。
采用逆转录-聚合酶链反应（RT-PCR）和免疫组织化学方法检测 12 个牙胚、47 个成釉细胞瘤和 6 个恶性成釉细胞瘤组织标本中 CD133、Bmi-1 和 ABCG2 的表达。
所有正常和肿瘤性牙源性组织样本中均检测到 CD133、Bmi-1 和 ABCG2 的 mRNA 表达。在牙胚、成釉细胞瘤和转移的成釉细胞瘤以及成釉细胞癌和透明细胞牙源性癌中，CD133 和 Bmi-1 的免疫组织化学反应在紧邻牙胚、成釉细胞瘤和转移的成釉细胞瘤以及成釉细胞癌和透明细胞牙源性癌基底膜的牙源性上皮细胞中可见，大多数肿瘤细胞呈 CD133 和 Bmi-1 反应性。恶性成釉细胞瘤中 CD133 免疫反应性水平明显高于牙胚和成釉细胞瘤。在一些成釉细胞瘤中检测到牙源性上皮成分的 ABCG2 免疫反应性，但在牙胚组织中未检测到。颗粒细胞成釉细胞瘤中的一些颗粒状肿瘤细胞表达 ABCG2。恶性成釉细胞瘤中 ABCG2 免疫反应性水平明显高于牙胚。
牙胚和成釉细胞瘤中 CD133、Bmi-1 和 ABCG2 的表达表明，干细胞相关分子可能控制牙源性组织的维持。这些分子的表达可能参与牙源性上皮的肿瘤发生、细胞分化和恶性潜能。
