Kalogirou Eleni-Marina, Lekakis Georgios, Petroulias Aristodimos, Chavdoulas Konstantinos, Zogopoulos Vasileios L, Michalopoulos Ioannis, Tosios Konstantinos I
Faculty of Health and Rehabilitation Sciences, Metropolitan College, 10672 Athens, Greece.
School of Dentistry, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Genes (Basel). 2023 Aug 30;14(9):1735. doi: 10.3390/genes14091735.
Stem cells have been associated with self-renewing and plasticity and have been investigated in various odontogenic lesions in association with their pathogenesis and biological behavior. We aim to provide a systematic review of stem cell markers' expression in odontogenic tumors and cysts.
The literature was searched through the MEDLINE/PubMed, EMBASE via OVID, Web of Science, and CINHAL via EBSCO databases for original studies evaluating stem cell markers' expression in different odontogenic tumors/cysts, or an odontogenic disease group and a control group. The studies' risk of bias (RoB) was assessed via a Joanna Briggs Institute Critical Appraisal Tool. Meta-analysis was conducted for markers evaluated in the same pair of odontogenic tumors/cysts in at least two studies.
29 studies reported the expression of stem cell markers, e.g., SOX2, OCT4, NANOG, CD44, ALDH1, BMI1, and CD105, in various odontogenic lesions, through immunohistochemistry/immunofluorescence, polymerase chain reaction, flow cytometry, microarrays, and RNA-sequencing. Low, moderate, and high RoBs were observed in seven, nine, and thirteen studies, respectively. Meta-analysis revealed a remarkable discriminative ability of SOX2 for ameloblastic carcinomas or odontogenic keratocysts over ameloblastomas.
Stem cells might be linked to the pathogenesis and clinical behavior of odontogenic pathologies and represent a potential target for future individualized therapies.
干细胞与自我更新和可塑性相关，并且已在各种牙源性病变中就其发病机制和生物学行为进行了研究。我们旨在对干细胞标志物在牙源性肿瘤和囊肿中的表达进行系统综述。
通过MEDLINE/PubMed、通过OVID的EMBASE、科学网以及通过EBSCO数据库的CINHAL检索文献，以查找评估干细胞标志物在不同牙源性肿瘤/囊肿、或牙源性疾病组与对照组中表达的原始研究。通过乔安娜·布里格斯研究所批判性评价工具评估研究的偏倚风险（RoB）。对至少两项研究中在同一对牙源性肿瘤/囊肿中评估的标志物进行荟萃分析。
29项研究通过免疫组织化学/免疫荧光、聚合酶链反应、流式细胞术、微阵列和RNA测序报告了干细胞标志物，例如SOX2、OCT4、NANOG、CD44、ALDH1、BMI1和CD105在各种牙源性病变中的表达。分别在7项、9项和13项研究中观察到低、中和高RoB。荟萃分析显示，与成釉细胞瘤相比，SOX2对成釉细胞癌或牙源性角化囊肿具有显著的鉴别能力。
干细胞可能与牙源性病变的发病机制和临床行为有关，并且是未来个体化治疗的潜在靶点。
