Bossé Janine T, Sinha Sunita, Schippers Timo, Kroll J Simon, Redfield Rosemary J, Langford Paul R
Molecular Infectious Disease Group, Department of Paediatrics, Imperial College London, London, UK.
FEMS Microbiol Lett. 2009 Sep;298(1):124-30. doi: 10.1111/j.1574-6968.2009.01706.x. Epub 2009 Jun 26.
We have identified a highly transformable strain of Actinobacillus pleuropneumoniae whose competence is regulated by the competence-activator Sxy as in other Pasteurellaceae. Other strains were poorly transformable or nontransformable. The genomes of two poorly transformable strains contain intact sets of competence genes. Moreover, we show that the low competence of one of these strains is not due to an inability to induce sxy expression or to a defect in Sxy function, suggesting that some other component of the competence system is defective. Although the A. pleuropneumoniae sxy gene has only 24% identity to its Haemophilus influenzae homologue, both genes fully complemented an H. influenzae sxy knockout, demonstrating that Sxy function is conserved throughout the Pasteurellaceae.
我们已鉴定出胸膜肺炎放线杆菌的一种高度可转化菌株，其感受态如同其他巴斯德氏菌科细菌一样，受感受态激活因子Sxy调控。其他菌株的可转化性较差或不可转化。两个可转化性较差的菌株基因组含有完整的感受态基因集。此外，我们发现其中一个菌株的低感受态并非由于无法诱导sxy表达或Sxy功能缺陷，这表明感受态系统的其他某些成分存在缺陷。尽管胸膜肺炎放线杆菌的sxy基因与其流感嗜血杆菌同源基因仅有24%的同一性，但这两个基因都能完全互补流感嗜血杆菌的sxy基因敲除，表明Sxy功能在整个巴斯德氏菌科中是保守的。
