Lakins M A, Marrison J L, O'Toole P J, van der Woude M W
Centre of Immunology and Infection, Department of Biology, University of York and The Hull York Medical School, York YO10 5YW, UK.
J Microsc. 2009 Aug;235(2):128-37. doi: 10.1111/j.1365-2818.2009.03190.x.
Biofilms are an important element of the natural ecosystems but can be detrimental in health care and industrial settings. To improve our ability to combat biofilms, we need to understand the processes that facilitate their formation and dispersal. One approach that has proven to be invaluable is to image biofilms as they grow. Here we describe tools and protocols to visualize biofilms with multiphoton laser scanning microscopy, compare this with single photon laser scanning confocal microscopy and highlight best working procedures. Furthermore, we describe how with multiphoton laser scanning microscopy the laser can be used to manipulate the biofilm, specifically to achieve localized bleaching, killing or ablation within the biofilm biomass. These applications open novel ways to study the dynamics of biofilm formation, regeneration and dispersal.
生物膜是自然生态系统的重要组成部分，但在医疗保健和工业环境中可能有害。为了提高我们对抗生物膜的能力，我们需要了解促进其形成和扩散的过程。一种已被证明非常有价值的方法是在生物膜生长时对其进行成像。在这里，我们描述了使用多光子激光扫描显微镜观察生物膜的工具和方案，将其与单光子激光扫描共聚焦显微镜进行比较，并突出最佳工作程序。此外，我们还描述了如何利用多光子激光扫描显微镜，通过激光来操纵生物膜，特别是在生物膜生物质中实现局部漂白、杀灭或消融。这些应用为研究生物膜形成、再生和扩散的动力学开辟了新途径。
