Dept. of Biology, Georgia State University, Atlanta, GA 30303, USA.
J Microbiol Methods. 2010 May;81(2):101-7. doi: 10.1016/j.mimet.2010.02.001. Epub 2010 Feb 10.
Agarose was used to stabilize fragile biofilms cultivated in parallel plate flow cells prior to imaging by confocal laser scanning microscopy. An essential element to the success of the procedure was the application of a ceramic heat pad to the flow cell to maintain agarose fluidity until the biofilm was enveloped. Quantitative digital image analysis demonstrated the effectiveness of this technique for generating reproducible measurements of a three-dimensional biofilm structure. The described method will also benefit researchers who transport their flow cell-cultivated biofilms to a core facility for imaging.
琼脂糖被用于在使用共聚焦激光扫描显微镜对平行板流动池内培养的脆弱生物膜进行成像之前稳定生物膜。该方法成功的关键要素是在流动池上施加陶瓷加热垫，以保持琼脂糖的流动性，直至生物膜被包裹。定量数字图像分析证明了该技术在生成可重复的三维生物膜结构测量方面的有效性。所描述的方法也将使那些将流动池培养的生物膜运输到核心设施进行成像的研究人员受益。
