Simon Judy, Stoelken Gunda, Rienks Michael, Rennenberg Heinz
Institute of Forest Botany and Tree Physiology, University of Freiburg, Freiburg, Germany.
FEBS Lett. 2009 Sep 3;583(17):2907-10. doi: 10.1016/j.febslet.2009.07.052. Epub 2009 Aug 4.
The gas phase of the soil plays an important role in plant growth and development. We investigated the effect of rhizospheric NO as a signalling compound for N uptake of beech roots. Following exposure to NO, ammonium and glutamine uptake into roots were determined using (15)N-labelling, and gene expression of selected transporters was analysed by quantitative real-time PCR. Uptake of both N sources increased significantly with elevated NO concentration. However, with one exception, this increase was not reflected in up-regulation of expression of the respective transporters.
土壤气相在植物生长发育中起着重要作用。我们研究了根际一氧化氮（NO）作为山毛榉根氮吸收信号化合物的作用。在暴露于NO后，使用（15）N标记法测定根对铵和谷氨酰胺的吸收，并通过定量实时聚合酶链反应分析所选转运蛋白的基因表达。随着NO浓度升高，两种氮源的吸收均显著增加。然而，除了一个例外，这种增加并未反映在相应转运蛋白表达的上调上。
