Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):914-918. doi: 10.1099/ijs.0.014506-0. Epub 2009 Aug 6.
A novel actinobacterium, strain P30(T), was isolated from jeotgal, a traditional Korean fermented seafood. Cells were aerobic, Gram-positive, non-motile and coccoid. Optimal growth occurred at 30-37 degrees C, at pH 8-9 and in the presence of 0-2 % (w/v) NaCl. Based on 16S rRNA gene sequence analysis, strain P30(T) was phylogenetically closely related to Kocuria carniphila, Kocuria gwangalliensis, Kocuria rhizophila, Kocuria marina, Kocuria rosea and K. varians with levels of similarity of 98.6, 98.2, 98.1, 97.4, 97.3 and 97.3 %, respectively, to the type strains of these species. Levels of DNA-DNA relatedness between strain P30(T) and the type strains of K. carniphila, K. rhizophila, K. marina, K. rosea and K. varians were 37, 43, 37, 25 and 17 %, respectively. The predominant menaquinone of strain P30(T) was MK-7. Major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and iso-C(16 : 0). The genomic DNA G+C content of strain P30(T) was 70.2 mol%. Based on these data, strain P30(T) is considered to represent a novel species of the genus Kocuria, for which the name Kocuria atrinae sp. nov. is proposed. The type strain is P30(T) (=KCTC 19594(T)=JCM 15914(T)).
一株新的放线菌,菌株 P30(T),从韩国传统发酵海鲜 jeotgal 中分离得到。细胞为需氧、革兰氏阳性、非运动和球形。最佳生长条件为 30-37°C、pH8-9 和 0-2%(w/v)NaCl。根据 16S rRNA 基因序列分析,菌株 P30(T)与 Kocuria carniphila、Kocuria gwangalliensis、Kocuria rhizophila、Kocuria marina、Kocuria rosea 和 K. varians 密切相关,与这些种的模式菌株的相似度分别为 98.6%、98.2%、98.1%、97.4%、97.3%和 97.3%。菌株 P30(T)与 K. carniphila、K. rhizophila、K. marina、K. rosea 和 K. varians 的模式菌株之间的 DNA-DNA 同源性分别为 37%、43%、37%、25%和 17%。菌株 P30(T)的主要menaquinone 为 MK-7。主要细胞脂肪酸为 anteiso-C(15:0)、iso-C(15:0)和 iso-C(16:0)。菌株 P30(T)的基因组 DNA G+C 含量为 70.2 mol%。基于这些数据,菌株 P30(T)被认为代表了 Kocuria 属的一个新种,建议将其命名为 Kocuria atrinae sp. nov.。模式菌株为 P30(T)=KCTC 19594(T)=JCM 15914(T)。