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产盐丝菌属的一个新种,从盐腌海鲜中分离得到。

Kocuria salsicia sp. nov., isolated from salt-fermented seafood.

机构信息

Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.

KRIBB, University of Science & Technology, 52 Eoeun-dong, Daejeon 305-333, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):286-289. doi: 10.1099/ijs.0.021469-0. Epub 2010 Mar 12.

Abstract

Strain 104(T) was isolated from a traditional salt-fermented seafood in Korea. It was a Gram-positive, non-motile, coccus-shaped bacterium. It formed lemon-yellow, opaque colonies that were circular with entire margins. Optimal growth occurred at 30-37 °C, pH 7-8 and in the presence of 0-2 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences from strain 104(T) and reference species of the genus Kocuria indicated that strain 104(T) formed an independent line. The G+C content of the chromosomal DNA was 60.6 mol%. MK-7 was the major menaquinone and the predominant fatty acids were anteiso-C(15 : 0) (76.7 %), anteiso-C(17 : 0) (10.9 %) and iso-C(16 : 0) (4.5 %). Strain 104(T) was most closely related to Kocuria rhizophila TA68(T) (98.9 % 16S rRNA gene sequence similarity). The DNA-DNA hybridization value between strain 104(T) and K. rhizophila TA68(T) was 14.1±3.4 %. On the basis of this polyphasic taxonomic analysis, strain 104(T) appears to represent a novel species in the genus Kocuria. The name Kocuria salsicia sp. nov. is proposed. The type strain is 104(T) (=KACC 21128(T)=JCM 16361(T)).

摘要

104(T) 株从韩国传统的盐发酵海产品中分离得到。它是革兰氏阳性、非运动、球形细菌。它形成柠檬黄色、不透明的圆形菌落,边缘整齐。最佳生长条件为 30-37°C、pH7-8 和 0-2%(w/v)NaCl。菌株 104(T) 和属 Kocuria 参考种的 16S rRNA 基因序列的系统发育分析表明,菌株 104(T) 形成一个独立的谱系。染色体 DNA 的 G+C 含量为 60.6 mol%。MK-7 是主要的menaquinone,主要脂肪酸为 anteiso-C(15:0)(76.7%)、anteiso-C(17:0)(10.9%)和 iso-C(16:0)(4.5%)。菌株 104(T) 与 Kocuria rhizophila TA68(T)(98.9% 16S rRNA 基因序列相似度)最为密切相关。菌株 104(T) 和 K. rhizophila TA68(T) 之间的 DNA-DNA 杂交值为 14.1±3.4%。基于这种多相分类学分析,菌株 104(T) 似乎代表了 Kocuria 属中的一个新种。建议使用 Kocuria salsicia sp. nov. 这个新名称。其模式菌株为 104(T)(=KACC 21128(T)=JCM 16361(T))。

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