Parajó Yolanda, Malina Jaroslav, Meistermann Isabelle, Clarkson Guy J, Pascu Mirela, Rodger Alison, Hannon Michael J, Lincoln Per
Department of Chemistry and Bioscience, Chalmers University of Technology, Gothenburg, Sweden.
Dalton Trans. 2009 Jul 7(25):4868-74. doi: 10.1039/b822039e. Epub 2009 May 7.
Triple helical di-iron complexes, readily prepared through interaction of iron(II) ions with imine-based ligands, are cylinder-shaped tetracations comparable in size and shape to common protein DNA recognition units. They have a strong tendency to coil DNA, and have recently been found to induce formation of three-way junctions in palindromic oligonucleotides. To introduce potential H bond acceptor units onto the surface of triple-helicates, new iron(II) complexes have been synthesized in which the central linking unit in the bridging ligand is S or O, instead of CH(2). The DNA binding properties of these new metallo-helicates were studied using UV-vis spectroscopy and circular and linear dichroism. Results show that the three iron triple helicates bind the DNA in a similar way but that the stability of the triple helicate unit is decreased with the O linked bridging ligand.
通过铁（II）离子与亚胺基配体相互作用易于制备的三螺旋二铁配合物是圆柱状的四价阳离子，其大小和形状与常见的蛋白质-DNA识别单元相当。它们具有强烈的使DNA卷曲的倾向，并且最近发现能诱导回文寡核苷酸中三链交联的形成。为了在三螺旋体表面引入潜在的氢键受体单元，已合成了新的铁（II）配合物，其中桥连配体中的中心连接单元是S或O，而不是CH(2)。使用紫外可见光谱、圆二色光谱和线性二色光谱研究了这些新的金属螺旋体的DNA结合特性。结果表明，三种铁三螺旋体以相似的方式结合DNA，但三螺旋体单元的稳定性随着O连接的桥连配体而降低。
