Zhou Cheng, Wen Xiang-Hua
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China.
Huan Jing Ke Xue. 2009 Jun 15;30(6):1797-801.
To operate the white-rot fungal reactor under non-sterile conditions has important significance for its practical applications. Ozone (0.0144 mg/min) was selected as the bactericide to control the bacterial contamination in a white-rot fungal reactor. The control effect on bacteria, enzyme production, pH variation and decolorization of a recalcitrant dye (acid blue 45) in the reactor were studied during continuously operating the reactor. It was found that, the contamination bacteria were controlled at under a number of 1 x 10(5)-1 x 10(6) CFU/mL and the inactivation rate of contaminating bacteria in white-rot fungal reactor was close to 99%. The Phanerochaete chrysosporium continuously produced MnP with the maximum residual activity of 50 U/L and degraded the acid blue 45 with a rate of 40%-80%. At around pH 6, the system still had the capability to continuously produce MnP and degrade the acid blue 45. To keep the growth of white-rot fungi and production of MnP for a long-term operation is a next step point to be solved.
在非无菌条件下运行白腐真菌反应器对其实际应用具有重要意义。选择臭氧（0.0144毫克/分钟）作为杀菌剂来控制白腐真菌反应器中的细菌污染。在反应器连续运行期间，研究了其对反应器中细菌的控制效果、酶的产生、pH值变化以及难降解染料（酸性蓝45）的脱色情况。结果发现，污染细菌被控制在1×10⁵ - 1×10⁶ CFU/毫升以下，白腐真菌反应器中污染细菌的灭活率接近99%。黄孢原毛平革菌持续产生锰过氧化物酶，最大残留活性为50 U/L，并以40% - 80%的速率降解酸性蓝45。在pH值约为6时，该系统仍有能力持续产生锰过氧化物酶并降解酸性蓝45。为使白腐真菌长期生长并持续产生锰过氧化物酶以实现长期运行是下一步需要解决的问题。
