Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan.
Environ Technol. 2011 Aug-Sep;32(11-12):1239-46. doi: 10.1080/09593330.2010.534820.
A novel indigenous strain, Phanerochaete chrysosporium IBL-03, with high manganese peroxidase (MnP) activities was used for decolorization of a reactive textile dye, Drimarine Blue K2R, which is used extensively in textile units of Pakistan. The initial experiment was run for seven days with 0.01% (w/v) dye solution prepared in Kirk's basal nutrient medium. Samples were removed after every 24 h and the extent of dye decolorization was determined at lambda(max) of the dye. The study revealed that P. chrysosporium caused 65% decolorization of Drimarine Blue K2RL in seven days. By process optimization, 97% colour removal could be achieved in three days using 0.005% (w/v) Drimarine Blue K2RL solution at pH 4.0 and 30 degrees C in defined Kirk's medium with 0.9% (w/v) molasses and 0.2% (w/v) ammonium dihydrogen phosphate added as carbon and nitrogen sources, respectively. Manganese peroxidase was found to be the major enzyme (560 IU/mL) involved in dye decolorization of Drimarine Blue K2RL by P. chrysosporium. The dye adsorption studies showed that the dye initially adsorbed on fungal mats disappeared later on, possibly by the action of MnP secreted by the fungus in secondary metabolism.
一种新型的本土菌株，白腐菌 IBL-03，具有较高的锰过氧化物酶（MnP）活性，用于对一种反应性纺织染料，翠蓝 K2R 进行脱色，这种染料在巴基斯坦的纺织厂中广泛使用。初始实验在 Kirk 基本营养培养基中用 0.01%（w/v）的染料溶液进行了七天。每隔 24 小时取出样品，并在染料的最大吸收波长处测定染料的脱色程度。研究表明，白腐菌在七天内使翠蓝 K2RL 染料脱色 65%。通过工艺优化，在 pH4.0 和 30°C 的条件下，在定义的 Kirk 培养基中，用 0.005%（w/v）的翠蓝 K2RL 溶液，添加 0.9%（w/v）的糖蜜和 0.2%（w/v）的磷酸二氢铵作为碳源和氮源，可在三天内实现 97%的颜色去除。锰过氧化物酶是白腐菌对翠蓝 K2RL 进行脱色的主要酶（560IU/mL）。染料吸附研究表明，染料最初吸附在真菌垫上，后来消失了，可能是真菌在二次代谢中分泌的 MnP 作用所致。
