Huang Ting-Lin, Xu Jin-Lan, Tang Zhi-Xin, Xiao Zhou-Qiang
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2009 Jun 15;30(6):1838-43.
In an experimental investigation of bioaugmentation products affected on the petroleum contaminated soil. The influence of the bioaugmentation products dose, injections and temperature on bioremediation were studied. The results showed that the degradation rate was related positively to the amount of inoculation, when the dose was increased to 0.6 mg x kg(-1), total petroleum hydrocarbon (TPH) degradation rate was 87% in 48 days. The results of GC-MS indicated that the dominant petroleum constituents in oil-contaminated raw soil were 82.1% n-alkane, 16% alkene and little of others hydrocarbons, such as carotane, alkylnaphthalenes, hopanes, and steranes. The peaks amount of GC profile decreased from 32 to 14 after 40 days of bioremediation, this result indicated that branched alkanes, alkene, and alkylnaphthalenes were thoroughly degraded, then line alkanes, hopanes, and steranes were left in soil. In addition, the longer part of n-alkane were degraded with rate relatively higher, while the residual fraction at the end of the test is shorter part of n-alkane because bacteria degraded the longer n-alkane to shorter. The shorter n-alkane concentration decreased with increasing inoculation. One time injection of bioaugmentation products into soil clearly improved the biodegradation efficiency higher than injection of bioaugmentation products in turn. Soil temperature also affected TPH degradation rate when it was 30 degrees C, TPH rate reached 80%, where as when it was 20 degrees C, the TPH rate was lower to 60%, which indicated higher temperature improved TPH degradation and accelerated bioremediation.
在一项关于生物强化产品对石油污染土壤影响的实验研究中，研究了生物强化产品剂量、注入方式和温度对生物修复的影响。结果表明，降解率与接种量呈正相关，当剂量增加到0.6 mg x kg(-1)时，48天内总石油烃（TPH）降解率为87%。气相色谱-质谱联用（GC-MS）结果表明，受污染原生土壤中石油的主要成分是82.1%的正构烷烃、16%的烯烃以及少量其他烃类，如胡萝卜烷、烷基萘、藿烷和甾烷。生物修复40天后，气相色谱图的峰数从32个减少到14个，这表明支链烷烃、烯烃和烷基萘被彻底降解，然后土壤中留下了直链烷烃、藿烷和甾烷。此外，正构烷烃中较长的部分降解速率相对较高，而试验结束时残留的部分是较短的正构烷烃，因为细菌将较长的正构烷烃降解为较短的。较短的正构烷烃浓度随着接种量的增加而降低。一次性将生物强化产品注入土壤明显提高了生物降解效率，高于依次注入生物强化产品的情况。土壤温度也影响TPH降解率，当温度为30℃时，TPH降解率达到80%，而当温度为20℃时，TPH降解率降至60%，这表明较高的温度提高了TPH降解并加速了生物修复。
