Zhang Ruiping, Liu Qiang, Li Jianding, Zhang Kun, Li Jing
Department of Surgery, First Hospital, Shanxi Medical University, Taiyuan Shanxi, 030001, P.R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Jul;23(7):851-5.
To explore the labeling efficiency and cellular viability of rabbit BMSCs labeled with different concentrations of superparamagnetic iron oxide (SPIO) particles, and to determine the feasibility of magnetically labeled stem cells with MR imaging.
The BMSCs were collected from iliac marrow of 10 adult rabbits (weighing 2.5-3.0 kg) and cultured. The SPIO-poly-L-lysine compound by different ratios mixed with medium, therefore, the final concentration of Fe2+ was 150 (group A), 100 (group B), 50 (group C) and 25 microg (group D) per mL, respectively, the 3rd generation BMSCs culture medium was added to lable; non-labeled cells served as a control (group E). MR imaging of cell suspensions was performed by using T1WI and T2WI sequences at a clinical 1.5 T MRI system.
BMSCs were efficiently labeled with SPIO, labeled SPIO particles were stained in all cytoplasms of groups A, B, C and D. With the increasing of Fe2+ concentration, blue dye particles increased. The staining result was negative in group E. The cell viability in groups A, B, C, D and E was 69.20% +/- 6.11%, 80.41% +/- 2.42%, 94.32% +/- 0.67%, 96.24% +/- 0.34% and 97.43% +/- 0.33%, respectively. There were statistically significant differences between groups A, B and groups C, D and E (P < 0.05), and between group A and group B (P < 0.05). T1WI images had no specific difference among 5 groups, T2WI images decreased significantly in groups A, B, C, decreased slightly in group D, and had little change in group E. The T2WI signal intensities of groups A, B, C, D and E were 23.37 +/- 6.21, 26.73 +/- 3.60, 29.63 +/- 2.82, 45.03 +/- 6.76 and 783.15 +/- 7.38, respectively, showing significant difference between groups A, B, C, D and group E (P < 0.05), and between groups A, B, C and group D (P < 0.05).
BMSCs can be easily and efficiently labeled by SPIO without interference on the cell viability in labeled concentration of 20-50 microg Fe2+ per mL. MRI visualization of SPIO labeled BMSCs is feasible, which may be critical for future experimental studies.
探讨不同浓度超顺磁性氧化铁（SPIO）颗粒标记兔骨髓间充质干细胞（BMSCs）的标记效率及细胞活力，并确定磁共振成像对干细胞进行磁性标记的可行性。
从10只成年兔（体重2.5 - 3.0 kg）的髂骨髓中采集BMSCs并进行培养。将不同比例的SPIO - 聚-L-赖氨酸复合物与培养基混合，使Fe2+的终浓度分别为每毫升150（A组）、100（B组）、50（C组）和25微克（D组），加入第3代BMSCs培养基进行标记；未标记的细胞作为对照（E组）。在临床1.5 T MRI系统上使用T1WI和T2WI序列对细胞悬液进行磁共振成像。
BMSCs被SPIO有效标记，A、B、C、D组所有细胞质中均可见标记的SPIO颗粒。随着Fe2+浓度的增加，蓝色染料颗粒增多。E组染色结果为阴性。A、B、C、D、E组细胞活力分别为69.20%±6.11%、80.41%±2.42%、94.32%±0.67%、96.24%±0.34%和97.43%±0.33%。A、B组与C、D、E组之间差异有统计学意义（P < 0.05），A组与B组之间差异有统计学意义（P < 0.05）。5组T1WI图像无特异性差异，A、B、C组T2WI图像信号明显降低，D组略有降低，E组变化不大。A、B、C、D、E组T2WI信号强度分别为23.37±6.21、26.73±3.60、29.63±2.82、45.03±6.76和783.15±7.38，A、B、C、D组与E组之间差异有统计学意义（P < 0.05），A、B、C组与D组之间差异有统计学意义（P < 0.05）。
在每毫升20 - 50微克Fe2+的标记浓度下，SPIO可轻松高效地标记BMSCs，且不影响细胞活力。SPIO标记的BMSCs的MRI可视化是可行的，这对未来的实验研究可能至关重要。
