Christmann J L, Grayson M J, Huang R C
Biochemistry. 1977 Jul 12;16(14):3250-6. doi: 10.1021/bi00633a032.
Vitellogenin, the only phosphoprotein detectable in the plasma of laying hens, is present at an approximate concentration of 1 mg/mL and can be isolated by chromatography on diethylaminoethylcellulose. Vitellogenin has a molecular weight of 235 000--240 000 and contains approximately 3% phosphorus by weight. Evidence that this protein is the precursor of phosvitins includes its ability to act as an acceptor for phosphate with a phosvitin specific kinase, the generation of a peptide similar to phosvitin by trypsinization, and the presence of distinctive peptides of multiple clustered phosphoserine upon partial acid hydrolysis. This partial sequence similarity between phosvitins and vitellogenin has not been previously reported. The phosphorus content and amino acid composition of vitellogenin are consistent with a model which contains two phosvitins and one lipovitellin. The total molecular weights of these proteins (28 000 + 34 000 + 170 000 = 232 000) are close to that of vitellogenin.
卵黄生成素是蛋鸡血浆中唯一可检测到的磷蛋白，其浓度约为1毫克/毫升，可通过二乙氨基乙基纤维素柱色谱法分离。卵黄生成素的分子量为235000 - 240000，按重量计约含3%的磷。该蛋白质是卵黄高磷蛋白前体的证据包括：它能作为卵黄高磷蛋白特异性激酶的磷酸盐受体；经胰蛋白酶消化可产生类似于卵黄高磷蛋白的肽段；部分酸水解后存在多个成簇磷酸丝氨酸的独特肽段。卵黄高磷蛋白和卵黄生成素之间的这种部分序列相似性此前尚未见报道。卵黄生成素的磷含量和氨基酸组成与包含两个卵黄高磷蛋白和一个卵黄脂磷蛋白的模型相符。这些蛋白质的总分子量（28000 + 34000 + 170000 = 232000）与卵黄生成素的分子量相近。
