鸡血清中的钙结合蛋白：卵黄生成素和白蛋白。

Calcium-binding proteins in serum of chickens: vitellogenin and albumin.

作者信息

Guyer R B, Grunder A A, Buss E G, Clagett C O

出版信息

Poult Sci. 1980 Apr;59(4):874-9. doi: 10.3382/ps.0590874.

Abstract

The Ca-binding proteins of hen serum were resolved by gel filtration in 45Ca buffer and were named CaBP(1) and CaBP(2). The major calcium-binding protein, CaBP(1), had a molecular size of 6.0 X 10(5) daltons and appears to be vitellogenin. Deeley et al. (1975) described vitellogenin as the precursor of lipovitellin and phosvitin, the Ca-binding proteins of egg yolk. The present paper demonstrates that "native" vitellogenin is a calcium-binding protein in the serum of laying chickens. The CaBP(2) co-eluted with and was immunologically identical to chicken serum albumin which also bound 45Ca. Data from elution profiles and phosphorus assays indicated that CaBP(1) and CaBP(2) were different from phosvitin and the Ca-binding proteins of the duodenum and uterus.

摘要

通过在含45Ca的缓冲液中进行凝胶过滤，分离出了母鸡血清中的钙结合蛋白，并将其命名为CaBP(1)和CaBP(2)。主要的钙结合蛋白CaBP(1)，分子量为6.0×10(5)道尔顿，似乎是卵黄生成素。迪利等人（1975年）将卵黄生成素描述为卵黄脂磷蛋白和卵黄高磷蛋白的前体，卵黄脂磷蛋白和卵黄高磷蛋白是蛋黄中的钙结合蛋白。本文证明“天然”卵黄生成素是产蛋鸡血清中的一种钙结合蛋白。CaBP(2)与鸡血清白蛋白共洗脱，且在免疫上与之相同，鸡血清白蛋白也能结合45Ca。洗脱曲线和磷分析的数据表明，CaBP(1)和CaBP(2)与卵黄高磷蛋白以及十二指肠和子宫的钙结合蛋白不同。