Protter A A, Wang S Y, Shelness G S, Ostapchuk P, Williams D L
Nucleic Acids Res. 1982 Aug 25;10(16):4935-50. doi: 10.1093/nar/10.16.4935.
A clone for vitellogenin, a major avian, estrogen responsive egg yolk protein, was isolated from the cDNA library of estrogen-induced rooster liver. Two forms of plasma vitellogenin, vitellogenin I (VTG I) and vitellogenin II (VTG II), distinguishable on the basis of their unique partial proteolysis maps, have been characterized and their corresponding hepatic precursor forms identified. We have used this criterion to specifically characterize which vitellogenin protein had been cloned. Partial proteolysis maps of BTG I and VTG II standards, synthesized in vivo, were compared to maps of protein synthesized in vitro using RNA hybrid-selected by the vitellogenin plasmid. Eight major digest fragments were found common to the in vitro synthesized vitellogenin and the VTG II standard while no fragments were observed to correspond to the VTG I map. A restriction map of the VTG II cDNA clone permits comparison to previously described cDNA and genomic vitellogenin clones.
从雌激素诱导的公鸡肝脏cDNA文库中分离出一种卵黄蛋白原（一种主要的禽类雌激素反应性卵黄蛋白）的克隆。已经鉴定出两种血浆卵黄蛋白原形式，即卵黄蛋白原I（VTG I）和卵黄蛋白原II（VTG II），它们可根据其独特的部分蛋白水解图谱区分开来，并确定了它们相应的肝脏前体形式。我们利用这一标准来具体鉴定克隆的是哪种卵黄蛋白原。将体内合成的BTG I和VTG II标准品的部分蛋白水解图谱与使用卵黄蛋白原质粒杂交选择的RNA体外合成的蛋白质图谱进行比较。发现体外合成的卵黄蛋白原与VTG II标准品有八个主要消化片段相同，而未观察到与VTG I图谱相对应的片段。VTG II cDNA克隆的限制性图谱允许与先前描述的cDNA和基因组卵黄蛋白原克隆进行比较。
