Potrebko Inna, Resurreccion Anna V A
Department of Food Science and Technology, The University of Georgia, Griffin, Georgia 30223-11797, USA.
J Agric Food Chem. 2009 Sep 9;57(17):7750-6. doi: 10.1021/jf900667d.
Peanuts are known to synthesize resveratrol, a stilbene phytoalexin, associated with cancer chemopreventive activity and cardioprotection. A modified HPLC method developed and validated to determine trans-resveratrol and trans-piceid concentrations in a single analysis run exhibited recoveries, precision, linearity, and limits of detection consistent with or better than those of methods previously reported in the literature. The effect of combined treatments, ultrasound (US) followed by varying UV light (UV) doses (three distances, three exposure times) on trans-resveratrol and trans-piceid concentrations in peanuts was determined. All nine US-UV treatments significantly increased trans-resveratrol contents from 0.03 microg/g, in untreated peanut control, to 2.10-4.73 microg/g and trans-piceid contents from 0.07 to 0.23-0.38 microg/g. Increases in trans-resveratrol were not influenced by UV distance and time. US and UV, individually, increased trans-resveratrol to 4.29 and 2.36 microg/g, respectively. The US and UV treatment combinations used did not exhibit synergistic effect compared to US treatments alone.
已知花生能合成白藜芦醇，一种芪类植物抗毒素，与癌症化学预防活性和心脏保护作用有关。所开发并验证的一种改进型高效液相色谱法，可在单次分析运行中测定反式白藜芦醇和反式云杉新苷的浓度，其回收率、精密度、线性和检测限与文献中先前报道的方法一致或更优。测定了联合处理（超声（US）后施加不同紫外线（UV）剂量（三个距离、三个照射时间））对花生中反式白藜芦醇和反式云杉新苷浓度的影响。所有九种超声 - 紫外线处理均显著提高了反式白藜芦醇含量，从未经处理的花生对照中的0.03微克/克提高到2.10 - 4.73微克/克，以及反式云杉新苷含量从0.07提高到0.23 - 0.38微克/克。反式白藜芦醇的增加不受紫外线距离和时间的影响。单独的超声和紫外线分别将反式白藜芦醇提高到4.29微克/克和2.36微克/克。与单独的超声处理相比，所使用的超声和紫外线处理组合未表现出协同效应。
