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非生物诱导子（超声）处理条件对卡莱西克卡拉西（L.）葡萄皮中反式白藜芦醇产量的优化

Optimization of an abiotic elicitor (ultrasound) treatment conditions on trans-resveratrol production from Kalecik Karası ( L.) grape skin.

作者信息

Erte Ebru, Vural Nilüfer, Mehmetoğlu Ülkü, Güvenç Afife

机构信息

Faculty of Engineering, Chemical Engineering Department, Ankara University, Dögol Street 06100 10th Year Campus, Beşevler/Ankara, Turkey.

出版信息

J Food Sci Technol. 2021 Jun;58(6):2121-2132. doi: 10.1007/s13197-020-04722-w. Epub 2020 Aug 24.

DOI:10.1007/s13197-020-04722-w
PMID:33967310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8076395/
Abstract

Grapes are known to synthesize resveratrol, a stilbene phytoalexin, associated with cancer chemopreventive activity and cardioprotection. The effect of ultrasound (US) abiotic elicitor treatments on trans-resveratrol content in Kalecik Karası fresh and frozen grape skin was determined. D-optimal point change design was used under RSM for the experimental design of US treatment. The optimization was solved with the help of the Pareto areas and the optimal input variable values were determined by the desirability function with fuzzy similar perceivable ratio method. The optimum conditions of US treatment for fresh grape skin were determined as follows: incubation time-24 h, US application method-(P01), US frequency-20 kHz, US treatment time-60 min and ultrasonic intensity (UI)-1.15 W cm. The trans-resveratrol content (0.18 ± 0.01 mg/g) in the untreated grape skin significantly increased with optimum US treatment (3.58 ± 0.08 mg/g), increasing production to 19.9 times.

摘要

众所周知，葡萄能合成白藜芦醇，一种芪类植物抗毒素，与癌症化学预防活性和心脏保护作用有关。本研究测定了超声（US）非生物诱导处理对卡莱吉克卡拉西鲜葡萄皮和冻葡萄皮中反式白藜芦醇含量的影响。在响应面法（RSM）下，采用D-最优混料设计对US处理进行实验设计。借助帕累托区域求解优化问题，并采用模糊相似感知比法通过合意性函数确定最佳输入变量值。鲜葡萄皮US处理的最佳条件如下：孵育时间24小时，US施加方式（P01），US频率20kHz，US处理时间60分钟，超声强度（UI）1.15W/cm。未经处理的葡萄皮中反式白藜芦醇含量（0.18±0.01mg/g）在最佳US处理（3.58±0.08mg/g）下显著增加，产量提高到19.9倍。

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