Kuz'min I V, Rybakov S S, Ivaniushchenkov V N, Bykova L A, Gulenkin V M
Arch Exp Veterinarmed. 1990;44(6):865-72.
This study was undertaken for the purpose of determining the primary structure of the 3' end gene of RNA polymerase of foot-and-mouth disease virus A22 550. Reported are isolation and purification of the virus, isolation of RNA, synthesis of cDNA, experience obtained from cloning as well as analysis of hybridisation and isolation of plasmid DNA. Nucleotide sequences, characterised by specific clones, were tested for potential needle structures. Also described are homology comparisons among FMD virus types A12, A10, O1, and C1.
本研究旨在确定口蹄疫病毒A22 550 RNA聚合酶3'端基因的一级结构。报告了病毒的分离与纯化、RNA的分离、cDNA的合成、克隆经验以及杂交分析和质粒DNA的分离。对由特定克隆表征的核苷酸序列进行了潜在针状结构测试。还描述了口蹄疫病毒A12、A10、O1和C1型之间的同源性比较。
