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[Cloning fragments of the RNA polymerase gene of an attenuated variant of the foot-and-mouth disease virus A22].

作者信息

Lomakina N F, Falina G M, Shcherbakov A V, Drygin V V, Driagalin N N, Bykova L A, Rybakov S S

出版信息

Mol Gen Mikrobiol Virusol. 1995 Apr-Jun(2):33-6.

PMID:7477032
Abstract

The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.

摘要

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