Böhm M, Gierschik P, Schnabel P, Erdmann E
Medizinische Klinik I der Universität München, Germany.
Cardioscience. 1990 Jun;1(2):109-17.
Cardiac beta-adrenoceptors and the alpha-subunits of the inhibitory guanine-nucleotide binding proteins (Gi alpha) were determined in myocardial biopsies and in larger samples of explanted hearts from patients with dilated cardiomyopathy. The myocardium of one non-failing heart was studied for comparison. There were fewer beta-adrenoceptors in right and left ventricular samples from hearts with dilated cardiomyopathy than in the non-failing myocardium. The number of beta-adrenoceptors determined by equilibrium saturation experiments closely correlated with their density as measured by a saturating concentration of the radioligand 125Iodocyanopindolol in myocardial biopsies. Using the 32P-ADP-ribosylation of a 40kDa membrane protein catalyzed by pertussis toxin, an increase of Gi alpha could also be shown in both myocardial biopsies and explanted hearts.
在扩张型心肌病患者的心肌活检组织以及更大样本的离体心脏中，测定了心脏β-肾上腺素能受体和抑制性鸟嘌呤核苷酸结合蛋白（Giα）的α亚基。研究了一颗非衰竭心脏的心肌作为对照。扩张型心肌病患者心脏的左右心室样本中的β-肾上腺素能受体比非衰竭心肌中的少。通过平衡饱和实验测定的β-肾上腺素能受体数量与心肌活检中用放射性配体125I-氰吲哚洛尔饱和浓度测得的密度密切相关。利用百日咳毒素催化的40kDa膜蛋白的32P-ADP-核糖基化，在心肌活检组织和离体心脏中也都显示出Giα增加。
