Clinical Department for Companion Animals and Horses, Clinic of Internal Medicine and Clinical Epidemiology of Equids, Veterinary University of Vienna, Vienna, Austria.
J Anim Physiol Anim Nutr (Berl). 2010 Aug 1;94(4):455-64. doi: 10.1111/j.1439-0396.2009.00929.x. Epub 2009 Aug 3.
The time-dependent changes in intramuscular amino acid (AA) levels caused by exercise and by feeding a protein/AA supplement were analysed in nine horses. Horses were submitted to a total of four standardized exercise tests (SETs). Amino acid concentrations were determined prior to, immediately after, 4 and 18 h after exercise. The experiment was subdivided into two consecutive periods of 3 weeks. In each period two SETs were performed. In the second period, horses were given a protein/AA supplement within 1 h after exercise. Significant changes in mean plasma AA levels similar to previous studies were noted to be time-dependent and to be associated with feeding the supplement. The intramuscular concentrations of the free AA in relation to pre-exercise levels showed significant time-dependent changes for alanine, asparagine, aspartate, citrulline, glutamine, glycine, isoleucine, leucine, methionine, serine, taurine, threonine, tyrosine and valine. Feeding the supplement significantly increased the 4 h post-exercise intramuscular concentration of alanine, isoleucine, methionine and tyrosine. At 18 h after exercise, apart from isoleucine and methionine, levels were still increased and also those of asparagine, histidine and valine in relation to none treatment. Hence, it was concluded that AA mixtures administered orally to horses within 1 h after exercise increased intramuscular AA pool.
研究人员分析了 9 匹马在运动和补充蛋白质/氨基酸（AA）后肌肉内 AA 水平的时变情况。这些马匹总共接受了 4 项标准化运动测试（SETs）。在运动前、运动后即刻、4 小时和 18 小时测定氨基酸浓度。实验分为两个连续的 3 周期，每个周期进行 2 次 SETs。在第二个周期，马匹在运动后 1 小时内给予蛋白质/AA 补充剂。研究发现，与之前的研究类似，血浆 AA 水平的均值随时间的变化呈显著相关性，并与补充剂的摄入有关。与运动前水平相比，游离 AA 的肌肉内浓度在时间上显示出显著的变化，其中包括丙氨酸、天冬酰胺、天冬氨酸、瓜氨酸、谷氨酰胺、甘氨酸、异亮氨酸、亮氨酸、蛋氨酸、丝氨酸、牛磺酸、苏氨酸、酪氨酸和缬氨酸。补充剂的摄入显著增加了运动后 4 小时的肌肉内丙氨酸、异亮氨酸和蛋氨酸浓度。在运动后 18 小时，除异亮氨酸和蛋氨酸外，与不进行处理相比，水平仍在增加，天冬酰胺、组氨酸和缬氨酸的水平也在增加。因此，研究人员得出结论，在运动后 1 小时内给马匹口服 AA 混合物会增加肌肉内 AA 库。
