Hogue B G, Nayak D P
Department of Microbiology and Immunology, UCLA School of Medicine 90024.
Adv Exp Med Biol. 1990;276:121-6. doi: 10.1007/978-1-4684-5823-7_18.
Cloned cDNA encoding the M protein of the porcine transmissible gastroenteritis coronavirus (TGEV) was introduced into a vaccinia virus to examine the function of the amino-terminal signal peptide. The M protein expressed by the recombinant virus was targeted to the Golgi region of infected cells, as is the M protein in cells infected with TGEV. The protein appeared not to undergo processing other than glycosylation. However, the vaccinia-expressed M protein was slightly larger than the protein found in TGEV-infected cells, suggesting that a difference in modification exists between the proteins.
将编码猪传染性胃肠炎冠状病毒（TGEV）M蛋白的克隆cDNA导入痘苗病毒，以检测氨基末端信号肽的功能。重组病毒表达的M蛋白靶向感染细胞的高尔基体区域，就像TGEV感染细胞中的M蛋白一样。该蛋白似乎除了糖基化外未经历其他加工。然而，痘苗病毒表达的M蛋白比TGEV感染细胞中发现的蛋白略大，这表明这两种蛋白在修饰上存在差异。
