Department of Pathology, State Key Laboratory of GI Cancer Biology, Xijing Hospital, Fourth Military Medical University, Shaanxi Province, PR China.
J Exp Clin Cancer Res. 2009 Aug 9;28(1):111. doi: 10.1186/1756-9966-28-111.
APMCF1 is a novel human gene first cloned from apoptotic MCF-7 cells. Our previous study found ectogenic APMCF1 could induce G1 arrest in hepatocarcinoma cell line HHCC. In order to search its broad expression profile for further understanding of its mechanism in tumor, we investigated a subcellular location of APMCF1 and performed an immunohistochemistry study including various tumor and normal tissues. Discovery from the expression characterization of AMPCF1 may have applicability in the analysis of its biological function in tumor.
We investigated subcellular localization of APMCF1 by transient transfection in green monkey kidney epithelial cells (COS-7) with a fusion protein vector pEGFP-APMCF1 and detected expression profile in a broad range of normal and malignant human tissues via tissue microarray (TMA) by immunohistochemistry with polyclonal antibody first produced in our laboratory.
EGFP-APMCF1 was generally localized in the cytoplasm of COS-7 cell. Positive staining of APMCF1 was found in liver, lung, breast, colon, stomach, esophagus and testis, exhibited a ubiquitous expression pattern while its expression was up-regulated in tumor tissues compared with corresponding normal tissues. Normal brain neuron cells also showed expression of APMCF1, but negative in gliocyte cells and glioma. Both the normal and tumor tissues of ovary were absent of APMCF1 expression. Positive immunostaining for APMCF1 with large samples in liver, colon, esophagus, lung and breast carcinomas were 96% (51/53), 80% (44/55), 57% (30/53), 58% (33/57) and 34% (16/47) respectively.
These results revealed a cytoplastic expression pattern of APMCF1 and up-regulated in tumour tissues suggesting APMCF1 may have potential relationship with oncogenesis. The data presented should serve as a useful reference for further studies of APMCF1 functions in tumorigenesis and might provide a potential anti-tumor target.
APMCF1 是一种从凋亡 MCF-7 细胞中克隆的新型人类基因。我们之前的研究发现外源性 APMCF1 可以诱导肝癌细胞系 HHCC 的 G1 期停滞。为了进一步了解其在肿瘤中的机制，我们搜索了 APMCF1 的广泛表达谱，进行了包括各种肿瘤和正常组织的免疫组织化学研究。对 APMCF1 表达特征的发现可能适用于分析其在肿瘤中的生物学功能。
我们通过瞬时转染绿色猴肾上皮细胞 (COS-7) 中的融合蛋白载体 pEGFP-APMCF1 来研究 APMCF1 的亚细胞定位，并通过免疫组织化学用我们实验室首次制备的多克隆抗体在组织微阵列 (TMA) 中检测广泛的正常和恶性人类组织中的表达谱。
EGFP-APMCF1 通常定位于 COS-7 细胞的细胞质中。在肝脏、肺、乳腺、结肠、胃、食管和睾丸中发现 APMCF1 的阳性染色，表现出普遍的表达模式，而在肿瘤组织中其表达水平高于相应的正常组织。正常脑神经元细胞也表达 APMCF1，但神经胶质细胞和神经胶质瘤中为阴性。卵巢的正常和肿瘤组织均无 APMCF1 表达。用大量样本进行免疫组化染色，在肝癌、结肠癌、食管癌、肺癌和乳腺癌中的阳性免疫染色率分别为 96%（51/53）、80%（44/55）、57%（30/53）、58%（33/57）和 34%（16/47）。
这些结果揭示了 APMCF1 的细胞质表达模式，并且在肿瘤组织中上调，表明 APMCF1 可能与肿瘤发生有潜在关系。所提供的数据应为进一步研究 APMCF1 在肿瘤发生中的功能提供有用的参考，并可能为潜在的抗肿瘤靶点提供参考。
