Shi Hui-Rong, Zhang Rui-Tao
Department of Obstetrics and Gynecology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, 450052, P.R. China.
Ai Zheng. 2009 Aug;28(8):882-5. doi: 10.5732/cjc.008.10417.
P53, P21WAF1 and CDK1 are key factors in the "P53 pathway" of G2/M phase DNA damage checkpoint in cell cycle. This study was to investigate the expression and significance of P53, P21WAF1 and CDK1 proteins in epithelial ovarian cancer.
The expression of P53, P21WAF1 and CDK1 proteins in 20 specimens of normal ovarian tissues, 20 specimens of benign epithelial ovarian tumor and 76 specimens of epithelial ovarian cancer was detected by immunohistochemistry. Their correlations to the clinicopathologic characteristics of epithelial ovarian cancer and their interrelationships were analyzed.
Significant differences were noted in the positive rates of P53, P21WAF1 and CDK1 proteins between epithelial ovarian cancer and normal ovarian tissues, benign ovarian tumors (P < 0.05). In epithelial ovarian cancer, up-regulation of P53 protein was associated with advanced FIGO stage and poor differentiation; down-regulation of P21WAF1 protein was associated with advanced FIGO stage; CDK1 showed no association with any clinicopathologic factors. P53 and CDK1 expression were negatively correlated to P21WAF1 expression (r = -0.388, P = 0.001; r = -0.282, P = 0.014); P53 expression was positively correlated to CDK1 expression (r = 0.263, P = 0.022).
P53 protein is related to the malignancy of epithelial ovarian cancer, P53 and P21WAF1 protein may be related to the malignant development of epithelial ovarian cancer. CDK1 detection may be helpful in the early diagnosis of epithelial ovarian cancer.
P53、P21WAF1和CDK1是细胞周期G2/M期DNA损伤检查点“P53途径”中的关键因子。本研究旨在探讨P53、P21WAF1和CDK1蛋白在上皮性卵巢癌中的表达及意义。
采用免疫组织化学法检测20例正常卵巢组织、20例良性上皮性卵巢肿瘤及76例上皮性卵巢癌组织中P53、P21WAF1和CDK1蛋白的表达，并分析其与上皮性卵巢癌临床病理特征的相关性及相互关系。
上皮性卵巢癌与正常卵巢组织、良性卵巢肿瘤之间P53、P21WAF1和CDK1蛋白阳性率差异有统计学意义（P<0.05）。在上皮性卵巢癌中，P53蛋白上调与国际妇产科联盟（FIGO）分期晚及低分化相关；P21WAF1蛋白下调与FIGO分期晚相关；CDK1与任何临床病理因素均无相关性。P53和CDK1的表达与P21WAF1的表达呈负相关（r=-0.388，P=0.001；r=-0.282，P=0.014）；P53的表达与CDK1的表达呈正相关（r=0.263，P=0.022）。
P53蛋白与上皮性卵巢癌的恶性程度有关，P53和P21WAF1蛋白可能与上皮性卵巢癌的恶性进展有关。检测CDK1可能有助于上皮性卵巢癌的早期诊断。
