Wei Xin, Lü Qing-jie, Sun Han-xue, Qi Ya-fei, Wang Jin-ou, Cao Cheng-cheng
Department of Pathology, Shengjing Hospital, China Medical University, Shenyang 110004, China.
Zhonghua Bing Li Xue Za Zhi. 2012 Feb;41(2):86-90.
To investigate the expressions of phosphorylated protein kinase B (p-AKT), phosphorylated glycogen synthase kinase 3β (p-GSK3β) and β-catenin proteins and to evaluate their relationship with the clinical pathological characteristics in epithelial tumors of the ovary.
The expression of p-AKT, p-GSK3β, and β-catenin was detected with immunohistochemical staining (EnVision method) in 10 cases of benign epithelial neoplasia, 10 cases of borderline epithelial neoplasia and 70 cases of ovarian carcinoma. The relationship of the expression of p-AKT, p-GSK3β and β-catenin with the clinical pathological features was analyzed.
The positive expression rates of p-AKT, p-GSK3β and β-catenin in epithelial ovarian carcinoma were 67.1% (47/70), 60.0% (42/70) and 71.4% (50/70), respectively. Compared to the results of benign and borderline epithelial neoplasia, the expression of the three proteins in carcinoma of the ovary was significantly different (all P < 0.05).Positive correlation was found between p-AKT and p-GSK3β, p-GSK3β and β-catenin, and p-AKT and β-catenin in epithelial ovarian carcinoma (r = 0.546, 0.581, 0.500, respectively; all P < 0.05). Compared to the results of benign and borderline epithelial neoplasia, the expression of p-AKT protein in epithelial ovarian carcinoma was significantly different (all P < 0.05). The expression of p-AKT was correlated with the differentiation of epithelial ovarian carcinoma (P < 0.05), but no relationship was found between its expression and histological classification and FIGO staging (P > 0.05). The expression of p-GSK3β and β-catenin in epithelial ovarian carcinoma were both higher than that in benign and borderline epithelial neoplasia (P < 0.05), and correlated with tumor differentiation and FIGO staging (P < 0.05), but no relationship were found between their expression with histological classification (P > 0.05).
Positive correlations are found between p-AKT, p-GSK3β and β-catenin in epithelial ovarian carcinoma. The activation of β-catenin is possibly correlated with inactivation of p-GSK3β that binds to p-AKT.
研究磷酸化蛋白激酶B（p-AKT）、磷酸化糖原合酶激酶3β（p-GSK3β）及β-连环蛋白的表达情况，并评估其与卵巢上皮性肿瘤临床病理特征的关系。
采用免疫组织化学染色（EnVision法）检测10例卵巢良性上皮性肿瘤、10例卵巢交界性上皮性肿瘤及70例卵巢癌组织中p-AKT、p-GSK3β及β-连环蛋白的表达情况，并分析p-AKT、p-GSK3β及β-连环蛋白表达与临床病理特征的关系。
卵巢上皮性癌中p-AKT、p-GSK3β及β-连环蛋白的阳性表达率分别为67.1%（47/70）、60.0%（42/70）和71.4%（50/70）。与卵巢良性及交界性上皮性肿瘤相比，卵巢癌中这三种蛋白的表达差异有统计学意义（均P<0.05）。卵巢上皮性癌中p-AKT与p-GSK3β、p-GSK3β与β-连环蛋白、p-AKT与β-连环蛋白之间均呈正相关（r值分别为0.546、0.581、0.500，均P<0.05）。与卵巢良性及交界性上皮性肿瘤相比，卵巢上皮性癌中p-AKT蛋白的表达差异有统计学意义（均P<0.05）。p-AKT的表达与卵巢上皮性癌的分化程度相关（P<0.05），但与组织学分类及国际妇产科联盟（FIGO）分期无关（P>0.05）。卵巢上皮性癌中p-GSK3β及β-连环蛋白的表达均高于卵巢良性及交界性上皮性肿瘤（P<0.05），且与肿瘤分化程度及FIGO分期相关（P<0.05），但与组织学分类无关（P>0.05）。
卵巢上皮性癌中p-AKT、p-GSK3β及β-连环蛋白之间呈正相关。β-连环蛋白的激活可能与结合p-AKT的p-GSK3β失活有关。
