Hu Yongfeng, Qin Fujun, Huang Limin, Sun Qianwen, Li Chen, Zhao Yu, Zhou Dao-Xiu
National Key Laboratory for Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China.
Biochem Biophys Res Commun. 2009 Oct 16;388(2):266-71. doi: 10.1016/j.bbrc.2009.07.162. Epub 2009 Aug 5.
Histone deacetylases (HDAC) are important in plant gene expression. Here we show that the expression of rice HDAC genes is both tissue/organ-specific, and most of them are responsive to drought or salt stresses. Over-expression of several rice HDACs did not produce any visible phenotype, whereas down-regulation of a few HDAC genes affected different developmental aspects. Specifically, down-regulation of HDA703 by amiRNA reduced rice peduncle elongation and fertility, while inactivation of a closely related homolog HDA710 by RNAi affected vegetative growth. HDA704 RNAi altered plant height and flag leaf morphology. Down-regulation of HDT702 led to the production of narrowed leaves and stems. These data suggest that rice HDAC genes may have divergent developmental functions compared with closely related homologs in Arabidopsis.
组蛋白去乙酰化酶（HDAC）在植物基因表达中起着重要作用。在此我们表明，水稻HDAC基因的表达具有组织/器官特异性，并且它们中的大多数对干旱或盐胁迫有响应。几个水稻HDAC的过表达未产生任何可见表型，而少数HDAC基因的下调影响了不同的发育方面。具体而言，amiRNA介导的HDA703下调降低了水稻穗轴伸长和育性，而RNAi介导的密切相关同源物HDA710失活影响了营养生长。HDA704 RNAi改变了株高和剑叶形态。HDT702的下调导致叶片和茎变窄。这些数据表明，与拟南芥中密切相关的同源物相比，水稻HDAC基因可能具有不同的发育功能。
