Division of Nanobiotechnology, School of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
N Biotechnol. 2009 Dec 31;26(6):269-76. doi: 10.1016/j.nbt.2009.07.011. Epub 2009 Aug 5.
In the present study, a magnetic bead-based detection approach for protein microarrays is described as an alternative approach to the commonly used fluorescence-based detection system. Using the bead-based detection approach with applied magnetic force, it was possible to perform the detection step more rapidly as a result of the accelerated binding between the captured analyte in the microspot and the detection antibody, which was coupled to the magnetic beads. The resulting strong opacity shift on the microspots could be recorded with an ordinary flatbed scanner. In the context of autoimmunity, a set of 24 serum samples was analyzed for the presence of antibodies against 12 autoantigens using standard fluorescence and magnetic bead-based detection methods. Dynamic range, sensitivity, and specificity were determined for both detection methods. We propose from our findings that the magnetic bead-based detection option provides a simplified and cost effective readout method for protein microarrays.
在本研究中，描述了一种基于磁珠的蛋白质微阵列检测方法，作为常用的荧光检测系统的替代方法。通过使用基于珠的检测方法和施加的磁场力，可以更快速地进行检测步骤，因为微点中捕获的分析物与偶联到磁珠上的检测抗体之间的结合得到了加速。在微点上可以记录到强烈的不透明度变化，并可以使用普通平板扫描仪进行记录。在自身免疫的背景下，使用标准的荧光和基于磁珠的检测方法分析了 24 份血清样本，以确定是否存在针对 12 种自身抗原的抗体。确定了两种检测方法的动态范围、灵敏度和特异性。我们的研究结果表明，基于磁珠的检测方法为蛋白质微阵列提供了一种简化且具有成本效益的读出方法。
