Schwenk Jochen M, Nilsson Peter
SciLifeLab Stockholm, KTH - Royal Institute of Technology, Tomtebodav, Sweden.
Methods Mol Biol. 2011;785:183-9. doi: 10.1007/978-1-61779-286-1_12.
With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the -commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.
随着亲和试剂收集量的不断增加，对其功能和结合特异性进行验证成为一项挑战。为满足这一日益增长的需求，已出现了小型化和并行化平台，以证实其对多种结合物支架的适用性。在常用系统中，平面微阵列长期以来一直是首选平台，但其他替代系统也不断涌现，其中一种基于颜色编码微珠，用于在悬浮液中创建阵列。后一种系统目前能够通过在单个实验中针对多达500种分析物分析多达384个样品来进行二维多重分析。虽然分析物参数在组成方面具有灵活性，但无需建立微阵列生产设施即可促进扩展筛选。
