Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, C. Postal 19046, 81531-990, Curitiba, PR, Brazil.
J Proteomics. 2009 Nov 2;73(1):50-6. doi: 10.1016/j.jprot.2009.07.012. Epub 2009 Aug 5.
Herbaspirillum seropedicae is an endophytic bacterium that associates with rice, sugarcane and other economically important crops. Secreted proteins play a key role in the plant-bacterial interaction. Using 2D electrophoresis and peptide mass fingerprint mass spectrometry, 63 protein spots representing 41 different secreted proteins were identified during growth of H. seropedicae under nitrogen-sufficient conditions. In silico analysis showed that 25.4% of the proteins had signal peptides and 15.9% were predicted to be non-classically secreted. Among the most abundant were flagellar components and ABC-type transport system proteins. Nine secreted proteins had also been identified in the cellular proteome, suggesting that they also play a role in the extracellular environment. No type III secreted proteins were detected by comparison of the wild type strain with an hrcN mutant strain.
希瓦氏菌属是一种内生细菌，与水稻、甘蔗和其他重要经济作物有关。分泌蛋白在植物-细菌相互作用中起着关键作用。在氮充足条件下生长时，使用 2D 电泳和肽质量指纹图谱质谱法，鉴定了 63 个蛋白斑点，代表 41 种不同的分泌蛋白。计算机分析表明，25.4%的蛋白质具有信号肽，15.9%的蛋白质被预测为非经典分泌。其中最丰富的是鞭毛成分和 ABC 型运输系统蛋白。在细胞蛋白质组中也鉴定出了 9 种分泌蛋白，这表明它们也在细胞外环境中发挥作用。通过比较野生型菌株和 hrcN 突变菌株，没有检测到 III 型分泌蛋白。
