巴西固氮螺菌蛋白质的二维蛋白质组参考图谱。
A two-dimensional proteome reference map of Herbaspirillum seropedicae proteins.
作者信息
Chaves Daniela Fojo Seixas, Ferrer Pércio Pereira, de Souza Emanuel Maltempi, Gruz Leonardo Magalhães, Monteiro Rose Adele, de Oliveira Pedrosa Fábio
机构信息
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil.
出版信息
Proteomics. 2007 Oct;7(20):3759-63. doi: 10.1002/pmic.200600859.
Herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. Here, we present a 2-D reference map for H. seropedicae. Using MALDI-TOF-MS we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. Seventeen hypothetical or conserved hypothetical ORFs were shown to code for true gene products. These data will support the genome annotation process and provide a basis on which to undertake comparative proteomic studies.
固氮螺菌是一种与水稻、甘蔗和小麦等重要经济作物相关的内生固氮菌。在此，我们展示了固氮螺菌的二维参考图谱。利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS），我们鉴定出205个斑点，代表173种不同的蛋白质，计算得出平均每个基因有1.18种蛋白质。17个假定的或保守的假定开放阅读框被证明编码真实的基因产物。这些数据将支持基因组注释过程，并为进行比较蛋白质组学研究提供基础。
Zotero 插件