MOV10作为一种新型端粒酶相关蛋白。

MOV10 as a novel telomerase-associated protein.

作者信息

Nakano Mariko, Kakiuchi Yasutaka, Shimada Yukiko, Ohyama Mika, Ogiwara Yasuko, Sasaki-Higashiyama Narie, Yano Naoko, Ikeda Fumi, Yamada Ei, Iwamatsu Akihiro, Kobayashi Kazuo, Nishiyama Kiyoto, Ichikawa Sohsuke, Kaji Kazuhiko, Ide Toshinori, Murofushi Hiromu, Murakami-Murofushi Kimiko

机构信息

Department of Biology, Faculty of Science, Ochanomizu University, Tokyo 112-8610, Japan.

出版信息

Biochem Biophys Res Commun. 2009 Oct 16;388(2):328-32. doi: 10.1016/j.bbrc.2009.08.002. Epub 2009 Aug 5.

DOI:10.1016/j.bbrc.2009.08.002

PMID:19665004

Abstract

A novel telomerase-associated protein was isolated from porcine testis. The 115-kDa protein, purified with telomerase activity, was molecular cloned using human cDNA library, and identified as MOV10. The expression levels of both MOV10 mRNA and MOV10 protein in cancer cells were 2-3 times higher than that of the normal cells, and MOV10 mRNA was highly expressed in human testis and ovary. The anti-MOV10 antibody precipitated the telomerase activity from cancer cell extracts, and inhibited the telomerase activity in vitro. Sf9-expressed MOV10 protein bound to G-rich strand of both single- and double-stranded telomere-sequenced DNA, but not to single C-rich strand. ChIP assay showed the binding of MOV10 to telomere region in vivo. These data suggest that MOV10 is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

摘要

从猪睾丸中分离出一种新型端粒酶相关蛋白。通过端粒酶活性纯化得到的115 kDa蛋白，利用人cDNA文库进行分子克隆，并鉴定为MOV10。癌细胞中MOV10 mRNA和MOV10蛋白的表达水平均比正常细胞高2至3倍，且MOV10 mRNA在人类睾丸和卵巢中高表达。抗MOV10抗体从癌细胞提取物中沉淀出端粒酶活性，并在体外抑制端粒酶活性。Sf9表达的MOV10蛋白与单链和双链端粒序列DNA的富含G链结合，但不与富含C的单链结合。染色质免疫沉淀分析显示MOV10在体内与端粒区域结合。这些数据表明，MOV10通过端粒酶蛋白与端粒DNA的相互作用参与端粒酶催化反应的进程。