Biochemistry Department, Moharam Bik, Alexandria University, Al-Hadary Mosque Street, Alexandria, Egypt.
Food Chem Toxicol. 2009 Nov;47(11):2735-41. doi: 10.1016/j.fct.2009.08.001. Epub 2009 Aug 6.
Gossypol displays anticancer behavior and anti-fertility in males. Male rats were treated with either gossypol acetic acid (GAA) or gossypol-iron complex (GIC). Serum alanine transaminase (ALT) activity elevated of GAA. However, GIC-treated animals showed a decrease in hepatic glutathione (GSH) content with increased malondialdehyde (MDA) content. Whereas, GSH-Px specific activity increased in GAA group. GAA and GIC induce significant increases in the hepatic NEFA with remarkable decrease in the total saturated fatty acids with a significant increase of PUFA. Lipid peroxidation is inhibited by gossypol, which shield lipids against oxidative damage. Phenols are oxidized to phenoxy radicals, which do not permit anti-oxidation due to resonance stabilization. GAA stimulate hydroxyl radicals (()OH) generation and DNA damage. GAA and GIC produce increase in lipid peroxidation as proved by a steep rise in thiobarbituric acid reactive species (TBARS). Controversy of specificity of TBARS towards compounds other than MDA was reported. If TBARS increased, more specific assay to be employed. Assay of lipid classes and fatty acids pattern, reveled the significance of the technique in assessment of lipid peroxidation in tissues. GAA and GIC were powerful inhibitors of lipid peroxidation and exhibit pro- and antioxidant behavior, with less toxicity of GIC.
棉酚具有抗癌和抗雄性生育能力。雄性大鼠用醋酸棉酚（GAA）或棉酚-铁复合物（GIC）处理。GAA 使血清丙氨酸转氨酶（ALT）活性升高。然而，GIC 处理的动物肝组织谷胱甘肽（GSH）含量降低，丙二醛（MDA）含量增加。GAA 组的 GSH-Px 比活性增加。GAA 和 GIC 诱导肝游离脂肪酸（NEFA）显著增加，总饱和脂肪酸显著减少，多不饱和脂肪酸（PUFA）显著增加。棉酚抑制脂质过氧化，保护脂质免受氧化损伤。酚类被氧化为苯氧自由基，由于共振稳定，不允许抗氧化。GAA 刺激羟自由基（()OH）的产生和 DNA 损伤。GAA 和 GIC 产生脂质过氧化增加，如硫代巴比妥酸反应性物质（TBARS）急剧增加所证明的那样。据报道，TBARS 对 MDA 以外的化合物的特异性存在争议。如果 TBARS 增加，应采用更特异的测定方法。脂质类和脂肪酸模式的测定揭示了该技术在组织脂质过氧化评估中的重要性。GAA 和 GIC 是脂质过氧化的有效抑制剂，表现出促氧化剂和抗氧化剂行为，且 GIC 的毒性较小。
