Wang Lin, Chen Ming, Luo Xuecai, Fan Yanan, Zheng Zai, He Zongqin, Yin Ruochun, Meng Tao, Xu Shuyang, Pan Yu, Su Jihu, Du Jiangfeng, Zhang Liang, Tian Xiaohe, Tian Yupeng, Chen Dongdong, Ge Honghua, Zhang Nannan, Li Ping
Research Center for Translational Medicine at Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.
School of Life Sciences, Anhui University, Hefei, China.
Front Chem. 2020 Dec 1;8:583176. doi: 10.3389/fchem.2020.583176. eCollection 2020.
The presence of the phenol gossypol has severely limited the utilization of cottonseed meal and oil in the food and animal feed industries. Highly efficient means of biodegradation of gossypol and an understanding of the cytotoxicity of its degradation products remain outside current knowledge and are of universal interest. In this work, we showed for the first time that laccase can catalyze the intramolecular annulation of the aldehyde and hydroxyl groups of gossypol for the -semiquinone radical and originate the released ·OH radical. It was further found that the oxidation of aldehyde groups significantly decreases reproductive toxicity and hepatotoxicity. These results indicate a novel detoxification pathway for gossypol and reveal the crucial role played by radical species in cyclization. This discovery could facilitate the development of safe, convenient, and low-cost industrial methods for the detoxification of cotton protein and oil resources.
棉籽酚的存在严重限制了棉籽粕和棉籽油在食品和动物饲料行业的应用。目前，高效生物降解棉酚的方法以及对其降解产物细胞毒性的了解仍属未知领域，受到广泛关注。在本研究中，我们首次表明漆酶可以催化棉酚醛基和羟基的分子内环化反应生成半醌自由基，并产生释放的·OH自由基。进一步研究发现，醛基的氧化显著降低了生殖毒性和肝毒性。这些结果表明了一种新的棉酚解毒途径，并揭示了自由基物种在环化反应中所起的关键作用。这一发现有助于开发安全、便捷且低成本的工业方法，用于棉籽蛋白和油脂资源的解毒。
