Ciechanover A, Gonen H
Department of Biochemistry, Rappaport Institute for Research in the Medical Sciences, Haifa, Israel.
Semin Cell Biol. 1990 Dec;1(6):415-22.
Degradation of proteins by the ubiquitin system involves two discrete steps. Initially, ubiquitin is covalently linked in an ATP-dependent mode to the protein substrate. The protein moiety of the conjugate is subsequently degraded by a specific protease into peptides and free amino acids with the release of free and reutilizable ubiquitin. The degradation process also requires energy. In this review we shall discuss the mechanisms involved in ubiquitin activation, selection of substrates for conjugation, and subsequent degradation of ubiquitin-conjugated proteins. In addition, we shall briefly summarize what is currently known of the role of the ubiquitin system in protein degradation in vitro and in vivo.
泛素系统介导的蛋白质降解包括两个独立步骤。首先，泛素以ATP依赖的方式共价连接到蛋白质底物上。随后，该缀合物的蛋白质部分被一种特异性蛋白酶降解为肽段和游离氨基酸，同时释放出游离的、可再利用的泛素。蛋白质降解过程也需要能量。在这篇综述中，我们将讨论泛素激活、缀合底物选择以及随后泛素缀合蛋白降解所涉及的机制。此外，我们还将简要总结目前已知的泛素系统在体外和体内蛋白质降解中的作用。
