Pereira José E, Costa Luís M, Cabrita António M, Couto Pedro A, Filipe Vítor M, Magalhães Luís G, Fornaro Michele, Di Scipio Federica, Geuna Stefano, Maurício Ana C, Varejão Artur S P
Department of Veterinary Sciences, CITAB, University of Trás-os-Montes e Alto Douro, P.O. Box 1013, 5001-801 Vila Real, Portugal.
Exp Neurol. 2009 Nov;220(1):71-81. doi: 10.1016/j.expneurol.2009.07.030. Epub 2009 Aug 7.
Currently, methylprednisolone sodium succinate (MPSS) is the standard treatment following acute spinal cord injury (SCI) as a consequence of the results obtained from the National Acute Spinal Cord Injury Studies. However, many have questioned the efficacy of MPSS because of its marginal effects. Additionally there has been criticism of both study design and statistical interpretation. The functional consequences of experimental SCI have been assessed in many ways. The purpose of this investigation was to determine the effects of MPSS vs. saline solution (SS) following moderate T10 contusion injury in rat. Functional recovery was evaluated using the 21-point Basso, Beattie and Bresnahan (BBB) locomotor recovery scale, the inclined plane, the beam walk, footprint analysis and the horizontal ladder. To optimize the precision and accuracy of functional results we examined the locomotion on a treadmill using three-dimensional (3D) analysis. Stereology was used to estimate the amount of damaged tissue. The results of the traditional functional methods showed that administration of the NASCIS dosage of MPSS following acute spinal cord contusion did not lead to any significant differences in the functional recovery of MPSS- vs. SS-treated animals. More importantly, the results of the 3D kinematic showed that the MPSS administration did not affect the flexion/extension of the hip, knee and ankle joints during the step cycle. Finally, stereological results revealed no statistically significant differences between the two experimental groups. Altogether, our results support data previously reported by several authors, suggesting that MPSS does not lead to improved functional outcome following experimental acute SCI.
目前，由于国家急性脊髓损伤研究得出的结果，甲泼尼龙琥珀酸钠（MPSS）是急性脊髓损伤（SCI）后的标准治疗药物。然而，由于其效果有限，许多人对MPSS的疗效提出了质疑。此外，对研究设计和统计解读都存在批评。实验性SCI的功能后果已通过多种方式进行评估。本研究的目的是确定在大鼠T10中度挫伤损伤后MPSS与生理盐水（SS）的效果。使用21分的Basso、Beattie和Bresnahan（BBB）运动恢复量表、斜面试验、横梁行走试验、足迹分析和水平梯试验来评估功能恢复情况。为了优化功能结果的精度和准确性，我们使用三维（3D）分析在跑步机上检查运动情况。体视学用于估计受损组织的数量。传统功能方法的结果表明，急性脊髓挫伤后给予NASCIS剂量的MPSS，在MPSS治疗组和SS治疗组动物的功能恢复方面没有导致任何显著差异。更重要的是，3D运动学结果表明，MPSS给药在步周期中不影响髋、膝和踝关节的屈伸。最后，体视学结果显示两个实验组之间没有统计学上的显著差异。总之，我们的结果支持了几位作者先前报道的数据，表明MPSS在实验性急性SCI后不会导致功能结果改善。
