Park Shin Yong, Kim Chong Han, Jeong Woo Hyuk, Lee Joon Ha, Seo Sook Jae, Han Yeon Soo, Lee In Hee
Department of Life Science, Hoseo University, Sechul-Ri, Baebang-Myun, Asan City, Chungnam-Do 336-795, South Korea.
Dev Comp Immunol. 2005;29(1):43-51. doi: 10.1016/j.dci.2004.06.001.
Two hemolymph proteins were isolated from the wax moth, Galleria mellonella, larvae by a two-step procedure consisting of acid extraction and reversed phase (RP)-HPLC. One was an apolipophorin III (apoLp-III) previously characterized as a lipopolysaccharide (LPS) binding protein in the hemolymph of G. mellonella. The other was confirmed to be a new protein with a molecular mass of 23,768.69 Da, referred to as Gm protein-24. The full-length cDNA of Gm protein-24 was cloned from the fat body. The cDNA structure showed that it is a 219-residues protein derived from the precursor of 236 amino acids. The effects of apoLp-III and Gm protein-24 have been tested on the insect humoral immunity. ApoLp-III enhanced the activity of antibacterial peptide such as cecropin but Gm protein-24 had no effect on cecropin activity. On the other hand, Gm protein-24 and apoLp-III were both involved in the activation of prophenoloxidase (PPO) cascade, which has been regarded as a critical immune reaction in insect hemolymph. Of note, the Gm protein-24 was a significantly stronger activator of PPO cascade than apoLp-III.
通过由酸提取和反相（RP）-高效液相色谱组成的两步法，从大蜡螟（Galleria mellonella）幼虫中分离出两种血淋巴蛋白。一种是载脂蛋白III（apoLp-III），之前已被鉴定为大蜡螟血淋巴中的脂多糖（LPS）结合蛋白。另一种被确认为一种分子量为23,768.69 Da的新蛋白，称为Gm蛋白-24。从脂肪体中克隆出Gm蛋白-24的全长cDNA。该cDNA结构表明它是一种由236个氨基酸的前体衍生而来的219个残基的蛋白。已经测试了apoLp-III和Gm蛋白-24对昆虫体液免疫的影响。ApoLp-III增强了抗菌肽如天蚕素的活性，但Gm蛋白-24对天蚕素活性没有影响。另一方面，Gm蛋白-24和apoLp-III都参与了酚氧化酶原（PPO）级联反应的激活，这被认为是昆虫血淋巴中的一种关键免疫反应。值得注意的是，Gm蛋白-24是比apoLp-III更强的PPO级联反应激活剂。
