Stewart Lucy R, Hwang Min Sook, Falk Bryce W
Department of Plant Pathology, University of California, Davis, One Shields Ave., Davis, CA 95616, USA.
Virus Res. 2009 Nov;145(2):293-9. doi: 10.1016/j.virusres.2009.07.021. Epub 2009 Aug 7.
Interactions of Lettuce infectious yellows virus (LIYV)-encoded proteins were tested by yeast-two-hybrid (Y2H) assays. LIYV-encoded P34, Hsp70h, P59, CP, CPm, and P26 were tested in all possible pairwise combinations. Interaction was detected only for the P26-P26 combination. P26 self-interaction domains were mapped using a series of N- and C-terminal truncations. Orthologous P26 proteins from the criniviruses Beet pseudoyellows virus (BPYV), Cucurbit yellow stunting disorder virus (CYSDV), and Lettuce chlorosis virus (LCV) were also tested, and each exhibited strong self-interaction but no interaction with orthologous proteins. Two small putative proteins encoded by LIYV RNA2, P5 and P9, were also tested for interactions with the six aforementioned LIYV proteins and each other. No interactions were detected for P5, but P9-P9 self-interaction was detected. P26- and P9-encoding genes are present in all described members of the genus Crinivirus, but are not present in other members of the family Closteroviridae. LIYV P26 has previously been demonstrated to induce a unique LIYV cytopathology, plasmalemma deposits (PLDs), but no role is yet known for P9.
通过酵母双杂交（Y2H）试验检测了生菜传染性黄化病毒（LIYV）编码蛋白之间的相互作用。对LIYV编码的P34、Hsp70h、P59、CP、CPm和P26进行了所有可能的两两组合检测。仅在P26 - P26组合中检测到相互作用。使用一系列N端和C端截短体对P26自相互作用结构域进行了定位。还检测了来自黄症病毒属的甜菜假黄化病毒（BPYV）、南瓜黄化矮缩病毒（CYSDV）和生菜褪绿病毒（LCV）的直系同源P26蛋白，它们各自均表现出强烈的自相互作用，但与直系同源蛋白无相互作用。还检测了由LIYV RNA2编码的两个假定小蛋白P5和P9与上述六种LIYV蛋白之间以及它们彼此之间的相互作用。未检测到P5的相互作用，但检测到P9 - P9自相互作用。P26和P9编码基因存在于黄症病毒属的所有已描述成员中，但在长线形病毒科的其他成员中不存在。LIYV P26先前已被证明可诱导独特的LIYV细胞病理学现象，即质膜沉积物（PLD），但P9的作用尚不清楚。
