Shandong Province Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
College of Plant Protection, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Taian 271018, China.
Viruses. 2023 Apr 18;15(4):990. doi: 10.3390/v15040990.
Tomato chlorosis virus (ToCV) severely threatens tomato production worldwide. P27 is known to be involved in virion assembly, but its other roles in ToCV infection are unclear. In this study, we found that removal of p27 reduced systemic infection, while ectopic expression of p27 promoted systemic infection of potato virus X in . We determined that catalases (SlCAT) can interact with p27 in vitro and in vivo and that amino acids 73 to 77 of the N-terminus of SlCAT represent the critical region for their interaction. p27 is distributed in the cytoplasm and nucleus, and its coexpression with SlCAT1 or SlCAT2 changes its distribution in the nucleus. Furthermore, we found that silencing of and can promote ToCV infection. In conclusion, p27 can promote viral infection by binding directly to inhibit anti-ToCV processes mediated by SlCAT1 or SlCAT2.
番茄褪绿病毒（ToCV）严重威胁着全球的番茄生产。已知 P27 参与病毒粒子的组装，但它在 ToCV 感染中的其他作用尚不清楚。在本研究中，我们发现去除 p27 会降低系统性感染，而 p27 的异位表达会促进马铃薯 X 病毒在 中的系统性感染。我们确定 SlCAT 中的过氧化氢酶（SlCAT）可以在体外和体内与 p27 相互作用，并且 SlCAT 的 N 端氨基酸 73 到 77 代表它们相互作用的关键区域。p27 分布在细胞质和细胞核中，其与 SlCAT1 或 SlCAT2 的共表达会改变其在细胞核中的分布。此外，我们发现 和 的沉默可以促进 ToCV 感染。总之，p27 可以通过直接结合来抑制 SlCAT1 或 SlCAT2 介导的抗 ToCV 过程，从而促进病毒感染。
