用于研究DNA聚合酶反应途径的技术。
Techniques used to study the DNA polymerase reaction pathway.
作者信息
Joyce Catherine M
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, P.O. Box 208114, New Haven, CT 06520-8114, USA.
出版信息
Biochim Biophys Acta. 2010 May;1804(5):1032-40. doi: 10.1016/j.bbapap.2009.07.021. Epub 2009 Aug 7.
Abstract
A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.
摘要
20多年前，利用化学淬灭方法建立了DNA聚合酶的最小反应途径。从那时起，人们对反应途径中的非共价步骤、涉及聚合酶或其DNA底物的构象变化产生了浓厚兴趣，这些变化可能在底物特异性中发挥作用。为了研究这些构象转变，人们设计了基于荧光的检测方法，所获得的结果为反应途径增添了新的细节。
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