表达双突变霍乱毒素A和B亚基的口服MucoRice诱导毒素特异性中和免疫。
Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity.
作者信息
Yuki Yoshikazu, Tokuhara Daisuke, Nochi Tomonori, Yasuda Hiroshi, Mejima Mio, Kurokawa Shiho, Takahashi Yuko, Kataoka Nobuhiro, Nakanishi Ushio, Hagiwara Yukari, Fujihashi Kohtaro, Takaiwa Fumio, Kiyono Hiroshi
机构信息
The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
出版信息
Vaccine. 2009 Oct 9;27(43):5982-8. doi: 10.1016/j.vaccine.2009.07.071. Epub 2009 Aug 7.
Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.
水稻表达的霍乱毒素B（CTB）亚基是一种无需冷链的口服疫苗，可有效诱导肠毒素中和免疫。我们制备了另一种基于水稻的疫苗MucoRice，它表达带有CTA和CTB亚基的无毒双突变霍乱毒素（dmCT）。蛋白质免疫印迹分析表明，MucoRice-dmCT具有多组分疫苗的形态。口服MucoRice-dmCT可诱导产生CTB特异性而非CTA特异性的血清IgG和黏膜IgA抗体，从而产生针对霍乱毒素的保护性免疫，而不会诱导产生水稻蛋白特异性抗体反应。MucoRice-dmCT的效力与MucoRice-CTB疫苗相当。MucoRice有潜力用作安全的多组分疫苗表达系统。
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