Martínez-Valenzuela Carmen, Gómez-Arroyo Sandra, Villalobos-Pietrini Rafael, Waliszewski Stefan, Calderón-Segura María Elena, Félix-Gastélum Rubén, Alvarez-Torres Armando
Departamento de Ciencias Biológicas, Universidad de Occidente, Boulevard Macario Gaxiola y Carretera Internacional, Los Mochis 81223, Sinaloa, Mexico.
Environ Int. 2009 Nov;35(8):1155-9. doi: 10.1016/j.envint.2009.07.010. Epub 2009 Aug 8.
Genotoxic damage was evaluated in 70 agricultural workers, 25 women and 45 men, exposed to pesticides in Las Grullas, Ahome, Sinaloa, Mexico, with an average of 7 years of exposure. The effect was detected through the sister chromatid exchanges (SCE) in lymphocytes of peripheral blood and micronuclei (MN) and other nuclear anomalies (NA) in buccal exfoliated cells. Also, the influence on cellular proliferation kinetics (CPK) was studied by means of the replication index (RI) and the cytotoxic effect was examined with the mitotic index (MI). The non-exposed group consisted of 70 other persons, 21 women and 47 men from the city of Los Mochis, Sinaloa, Mexico. Significant differences between the exposed and the non-exposed groups were observed in SCE, CPK, MI, MN and NA. Analysis of variance revealed that age, gender, smoking and alcohol consumption did not have a significant effect on genetic damage. However, there was a correlation between exposure time to pesticides and SCE frequency. These results could have been due to the exposure of workers to pesticides containing different chemical compounds. This study afforded valuable data to estimate the possible risk to health associated with pesticide exposure.
对墨西哥锡那罗亚州阿霍梅市拉斯格鲁亚斯的70名接触农药的农业工人（25名女性和45名男性）进行了遗传毒性损伤评估，他们的平均接触年限为7年。通过外周血淋巴细胞中的姐妹染色单体交换（SCE）以及颊部脱落细胞中的微核（MN）和其他核异常（NA）来检测这种影响。此外，通过复制指数（RI）研究了对细胞增殖动力学（CPK）的影响，并用有丝分裂指数（MI）检测了细胞毒性作用。未接触组由另外70人组成，来自墨西哥锡那罗亚州莫奇斯市，其中21名女性和47名男性。在SCE、CPK、MI、MN和NA方面，接触组和未接触组之间观察到显著差异。方差分析显示，年龄、性别、吸烟和饮酒对遗传损伤没有显著影响。然而，农药接触时间与SCE频率之间存在相关性。这些结果可能是由于工人接触了含有不同化合物的农药。这项研究提供了宝贵的数据，以估计与农药接触相关的可能健康风险。
