Ozalp Aliye, Barroso Begona
Exploratory Development Department, Astellas Pharma Global Development Europe, AC Leiderdorp, The Netherlands.
Anal Biochem. 2009 Dec 1;395(1):68-76. doi: 10.1016/j.ab.2009.08.005. Epub 2009 Aug 8.
A simple and rapid analytical method is described for the simultaneous quantitative analysis of three different N-acylethanolamides in human biological samples: anandamide (AEA), oleoylethanolamide (OEA), and palmitoylethanolamide (PEA). The method is based on a new hybrid solid phase extraction-precipitation technology followed by ultra-performance liquid chromatography/mass spectrometry (UPLC/MS) analysis using d(4)-AEA as the internal standard. The method is linear up to 100ng/ml with a limit of quantitation of 50pg/ml for AEA and 100pg/ml for OEA and PEA. Good reproducibility, accuracy, and precision were demonstrated during the method validation. Application of this new methodology to the analysis of clinical study samples is presented.
本文描述了一种简单快速的分析方法，用于同时定量分析人体生物样本中的三种不同的N-酰基乙醇胺：花生四烯酸乙醇胺（AEA）、油酰乙醇胺（OEA）和棕榈酰乙醇胺（PEA）。该方法基于一种新的混合固相萃取-沉淀技术，随后以d(4)-AEA作为内标进行超高效液相色谱/质谱（UPLC/MS）分析。该方法在高达100ng/ml时呈线性，AEA的定量限为50pg/ml，OEA和PEA的定量限为100pg/ml。在方法验证过程中证明了良好的重现性、准确性和精密度。本文还介绍了这种新方法在临床研究样本分析中的应用。
