采用电喷雾串联质谱法测定人血清中的花生四烯酸乙醇胺及其他脂肪酰基乙醇胺。
Determination of anandamide and other fatty acyl ethanolamides in human serum by electrospray tandem mass spectrometry.
作者信息
Schreiber Daniela, Harlfinger Stephanie, Nolden Brit M, Gerth Christoph W, Jaehde Ulrich, Schömig Edgar, Klosterkötter Joachim, Giuffrida Andrea, Astarita Giuseppe, Piomelli Daniele, Markus Leweke F
机构信息
Department of Psychiatry and Psychotherapy, University of Cologne, 50924 Cologne, Germany.
出版信息
Anal Biochem. 2007 Feb 15;361(2):162-8. doi: 10.1016/j.ab.2006.11.027. Epub 2006 Dec 20.
We developed a new selective liquid chromatography-electrospray ionization-tandem mass spectrometry method for the identification and quantification of anandamide (AEA), an endogenous cannabinoid receptor ligand, and other bioactive fatty acid ethanolamides (FAEs) in biological samples. Detection limit (0.025 pmol for AEA and 0.1 pmol for palmitoylethanolamide (PEA) and oleoylethanolamide (OEA)) and quantification limit (0.2 pmol for AEA and 0.4 pmol for OEA and PEA) were in the high fmol to low pmol range for all analytes. Linear correlations (r(2)=0.99) were observed in the calibration curves for standard AEA over the range of 0.025-25 pmol and for standard PEA and OEA over the range of 0.1-500 pmol. This method provides a time-saving and sensitive alternative to existing methods for the analysis of FAEs in biological samples.
我们开发了一种新的选择性液相色谱-电喷雾电离-串联质谱法，用于鉴定和定量生物样品中的花生四烯酸乙醇胺（AEA）、一种内源性大麻素受体配体，以及其他生物活性脂肪酸乙醇酰胺（FAE）。所有分析物的检测限（AEA为0.025皮摩尔，棕榈酰乙醇胺（PEA）和油酰乙醇胺（OEA）为0.1皮摩尔）和定量限（AEA为0.2皮摩尔，OEA和PEA为0.4皮摩尔）处于高飞摩尔至低皮摩尔范围。在0.025 - 25皮摩尔范围内的标准AEA校准曲线以及0.1 - 500皮摩尔范围内的标准PEA和OEA校准曲线中观察到线性相关性（r(2)=0.99）。该方法为生物样品中FAE的分析提供了一种省时且灵敏的现有方法替代方案。
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