Harrison P J, Barton A J, Najlerahim A, Pearson R C
Department of Anatomy, St. Mary's Hospital Medical School, London, UK.
Neuroreport. 1990 Oct;1(2):149-52. doi: 10.1097/00001756-199010000-00017.
In-situ hybridization (ISH) has been used to determine the distribution of the mRNA encoding a non-NMDA glutamatergic receptor subtype in rat and human brain. In the rat, signal is concentrated over neurons in hippocampus and cerebellum, with moderate labelling of neocortex and diencephalon. In human brain, a similar hippocampal and cerebellar distribution is seen, although with lower overall levels. Quantitative comparison between normal and Alzheimer's disease (AD) brain reveals a modest increase of this mRNA in AD subiculum and CA4 hippocampal field with no change in cerebellum. The significance of the increase is discussed in relation to other data suggesting glutamatergic involvement in AD.
原位杂交（ISH）已被用于确定编码非NMDA谷氨酸能受体亚型的mRNA在大鼠和人脑中的分布。在大鼠中，信号集中在海马体和小脑中的神经元上，新皮层和间脑有中度标记。在人脑中，观察到类似的海马体和小脑分布，尽管总体水平较低。正常脑与阿尔茨海默病（AD）脑之间的定量比较显示，AD海马下托和CA4海马区中这种mRNA有适度增加，而小脑无变化。结合其他表明谷氨酸能参与AD的数据讨论了这种增加的意义。
