School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
Oral Oncol. 2009 Dec;45(12):1032-6. doi: 10.1016/j.oraloncology.2009.06.007. Epub 2009 Aug 8.
Oral squamous cell carcinoma (OSCC) in Asians is highly associated with the abuse of areca (betel) chewing. There are several hundred million Asians who chew areca and are therefore at high risk of OSCC. Aberrance in cyclin D1 (CCND1) and/or cortactin (CTTN), which are localized on 11q13, seems to be critical events for the development of oral carcinogenesis. This study identified amplifications of CCND1 and CTTN by quantitative (Q)-PCR analysis in 50% and 45% of OSCC samples, respectively. Co-amplification of both genes was identified in 20% of tumors. Higher CTTN expression was associated with nodal metastasis of the OSCC, while the amplification of CCND1 was identified in 28% of oral brushed samples from areca chewers, who form a high risk group for OSCC. This study confirms the importance of alterations in CCND1 and CTTN with respect to areca-associated OSCC, and demonstrates that there is an early occurrence of amplification of these genes in the risk population. The non-invasive brushing sampling method coupling with Q-PCR analysis needs to be validated for use as an early detection system for gene copy changes, which should aid oral cancer prevention.
亚洲人口腔鳞状细胞癌 (OSCC) 与咀嚼槟榔高度相关。有几亿亚洲人咀嚼槟榔，因此患 OSCC 的风险很高。细胞周期蛋白 D1 (CCND1) 和/或皮质蛋白 (CTTN) 的异常，定位于 11q13，似乎是口腔癌发生发展的关键事件。本研究通过定量 (Q)-PCR 分析分别在 50%和 45%的 OSCC 样本中鉴定出 CCND1 和 CTTN 的扩增。在 20%的肿瘤中鉴定出这两个基因的共扩增。较高的 CTTN 表达与 OSCC 的淋巴结转移相关，而 CCND1 的扩增则发生在 28%的咀嚼槟榔者的口腔刷取样本中，这些人是 OSCC 的高危人群。本研究证实了 CCND1 和 CTTN 的改变在与槟榔相关的 OSCC 中的重要性，并表明这些基因在高危人群中很早就发生了扩增。与 Q-PCR 分析相结合的非侵入性刷取采样方法需要经过验证，以作为基因拷贝变化的早期检测系统，这将有助于口腔癌的预防。
