Luo W S, Guo Z G, Tang X L
Research Section of Pharmacology, Hu-nan Medical University, Changsha, China.
Zhongguo Yao Li Xue Bao. 1990 Sep;11(5):435-8.
Neutrophil infiltration in the ischemic porcine hearts was analyzed by spectrophotometrical assay of the neutrophil specific enzyme-myeloperoxidase (MPO). The results showed that neutrophil infiltration in the ischemic myocardium was increased with time. An increase of MPO activity per 1 g tissue from 1.3 +/- 0.8 to 4 +/- 3 IU was observed as early as 10 min after occlusion of the coronary artery. Three h after occlusion MPO activity increased to 8.4 +/- 1.3 IU, 7 times greater than that of normal tissue. One-h occlusion followed by 2-h reperfusion caused even higher neutrophil accumulation. MPO activity increased to 14 +/- 3 IU, 11 times greater than normal tissue. Pretreatment with verapamil to reperfusion hearts decreased MPO activity to 4.7 +/- 0.7 IU. In addition, verapamil completely eliminated the first episode of arrhythmia 5-10 min after occlusion. Our studies demonstrate that neutrophils can rapidly accumulate into the ischemic myocardium and suggest that the protective action of verapamil may in part due to its inhibition of neutrophil infiltration in the ischemic myocardium.
通过对中性粒细胞特异性酶髓过氧化物酶（MPO）进行分光光度测定，分析了缺血猪心脏中的中性粒细胞浸润情况。结果显示，缺血心肌中的中性粒细胞浸润随时间增加。早在冠状动脉闭塞10分钟后，每1克组织的MPO活性就从1.3±0.8国际单位增加到4±3国际单位。闭塞3小时后，MPO活性增加到8.4±1.3国际单位，比正常组织高7倍。闭塞1小时后再灌注2小时导致更高的中性粒细胞聚集。MPO活性增加到14±3国际单位，比正常组织高11倍。对再灌注心脏用维拉帕米预处理可使MPO活性降至4.7±0.7国际单位。此外，维拉帕米完全消除了闭塞后5 - 10分钟的首次心律失常发作。我们的研究表明，中性粒细胞可迅速积聚到缺血心肌中，并提示维拉帕米的保护作用可能部分归因于其对缺血心肌中中性粒细胞浸润的抑制作用。
