大肠杆菌偶氮还原酶AzoR参与对亲电子醌类引起的硫醇特异性应激的抗性。
The Escherichia coli azoreductase AzoR Is involved in resistance to thiol-specific stress caused by electrophilic quinones.
作者信息
Liu Guangfei, Zhou Jiti, Fu Q Shiang, Wang Jing
机构信息
Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental andBiological Science and Technology, Dalian University of Technology, Dalian 116024, China.
出版信息
J Bacteriol. 2009 Oct;191(20):6394-400. doi: 10.1128/JB.00552-09. Epub 2009 Aug 7.
Abstract
The physiological role of Escherichia coli azoreductase AzoR was studied. It was found that AzoR was capable of reducing several benzo-, naphtho-, and anthraquinone compounds, which were better substrates for AzoR than the model azo substrate methyl red. The DeltaazoR mutant displayed reduced viability when exposed to electrophilic quinones, which are capable of depleting cellular reduced glutathione (GSH). Externally added GSH can partially restore the impaired growth of the DeltaazoR mutant caused by 2-methylhydroquinone. The transcription of azoR was induced by electrophiles, including 2-methylhydroquinone, catechol, menadione, and diamide. A transcription start point was identified 44 bp upstream from the translation start point. These data indicated that AzoR is a quinone reductase providing resistance to thiol-specific stress caused by electrophilic quinones.
摘要
研究了大肠杆菌偶氮还原酶AzoR的生理作用。发现AzoR能够还原几种苯醌、萘醌和蒽醌化合物，这些化合物是比模型偶氮底物甲基红更好的AzoR底物。ΔazoR突变体在暴露于亲电醌时活力降低，亲电醌能够耗尽细胞内的还原型谷胱甘肽（GSH）。外部添加的GSH可以部分恢复由2-甲基对苯二酚引起的ΔazoR突变体受损的生长。azoR的转录由亲电试剂诱导，包括2-甲基对苯二酚、儿茶酚、甲萘醌和二酰胺。在翻译起始点上游44 bp处鉴定到一个转录起始点。这些数据表明AzoR是一种醌还原酶，可提供对亲电醌引起的硫醇特异性应激的抗性。
相似文献
1
The Escherichia coli azoreductase AzoR Is involved in resistance to thiol-specific stress caused by electrophilic quinones.
J Bacteriol. 2009 Oct;191(20):6394-400. doi: 10.1128/JB.00552-09. Epub 2009 Aug 7.
2
Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis.
Mol Microbiol. 2008 Mar;67(5):1108-24. doi: 10.1111/j.1365-2958.2008.06110.x. Epub 2008 Jan 16.
3
Genome-Scale Investigation of the Regulation of azoR Expression in Escherichia coli Using Computational Analysis and Transposon Mutagenesis.
Microb Ecol. 2024 May 1;87(1):63. doi: 10.1007/s00248-024-02380-5.
4
Characteristics of major Escherichia coli reductases involved in aerobic nitro and azo reduction.
J Appl Microbiol. 2013 Oct;115(4):1012-22. doi: 10.1111/jam.12294. Epub 2013 Jul 19.
5
Cloning and characterization of a Flavin-free oxygen-insensitive azoreductase from Klebsiella oxytoca GS-4-08.
Biotechnol Lett. 2019 Mar;41(3):371-378. doi: 10.1007/s10529-019-02647-9. Epub 2019 Jan 11.
6
Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms.
J Biol Chem. 2006 Jul 21;281(29):20567-76. doi: 10.1074/jbc.M513345200. Epub 2006 May 9.
7
Proteomic signatures uncover thiol-specific electrophile resistance mechanisms in Bacillus subtilis.
Expert Rev Proteomics. 2008 Feb;5(1):77-90. doi: 10.1586/14789450.5.1.77.
8
Expansion of substrate specificity and catalytic mechanism of azoreductase by X-ray crystallography and site-directed mutagenesis.
J Biol Chem. 2008 May 16;283(20):13889-96. doi: 10.1074/jbc.M710070200. Epub 2008 Mar 12.
9
Acceleration of azo dye decolorization by using quinone reductase activity of azoreductase and quinone redox mediator.
Bioresour Technol. 2009 Jun;100(11):2791-5. doi: 10.1016/j.biortech.2008.12.040. Epub 2009 Feb 8.
10
In-Situ Imaging of Azoreductase Activity in the Acute and Chronic Ulcerative Colitis Mice by a Near-Infrared Fluorescent Probe.
Anal Chem. 2019 Aug 20;91(16):10901-10907. doi: 10.1021/acs.analchem.9b02857. Epub 2019 Aug 9.
引用本文的文献
1
Protein profile of the Escherichia coli strain, BW25113, exposed to two novel iron-halide compounds: Fe(Hampy)2Cl4 and Fe(Hampy)2Br4.
Access Microbiol. 2025 Jan 28;7(1). doi: 10.1099/acmi.0.000783.v4. eCollection 2025.
2
Synthesis and Evaluation of Pseudoglucosinolates Releasing Isothiocyanates in the Presence of Azoreductases.
Chembiochem. 2025 Jun 16;26(12):e202500152. doi: 10.1002/cbic.202500152. Epub 2025 May 29.
3
Combination therapy delays antimicrobial resistance after adaptive laboratory evolution of .
Antimicrob Agents Chemother. 2025 Apr 2;69(4):e0148324. doi: 10.1128/aac.01483-24. Epub 2025 Mar 14.
4
An Innovative Inhibitor with a New Chemical Moiety Aimed at Biliverdin IXβ Reductase for Thrombocytopenia and Resilient against Cellular Degradation.
Pharmaceutics. 2024 Aug 30;16(9):1148. doi: 10.3390/pharmaceutics16091148.
5
Sequence similarity network analysis of drug- and dye-modifying azoreductase enzymes found in the human gut microbiome.
Arch Biochem Biophys. 2024 Jul;757:110025. doi: 10.1016/j.abb.2024.110025. Epub 2024 May 11.
6
Genome-Scale Investigation of the Regulation of azoR Expression in Escherichia coli Using Computational Analysis and Transposon Mutagenesis.
Microb Ecol. 2024 May 1;87(1):63. doi: 10.1007/s00248-024-02380-5.
7
Unravelling the physiological roles of mazEF toxin-antitoxin system on clinical MRSA strain by CRISPR RNA-guided cytidine deaminase.
J Biomed Sci. 2022 May 7;29(1):28. doi: 10.1186/s12929-022-00810-5.
8
Identification of Methicillin-Resistant (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection.
Antibiotics (Basel). 2022 Feb 26;11(3):316. doi: 10.3390/antibiotics11030316.
9
Octapeptin C4 and polymyxin resistance occur via distinct pathways in an epidemic XDR Klebsiella pneumoniae ST258 isolate.
J Antimicrob Chemother. 2019 Mar 1;74(3):582-593. doi: 10.1093/jac/dky458.
10
An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria.
Sci Rep. 2018 Oct 9;8(1):15021. doi: 10.1038/s41598-018-33291-4.
本文引用的文献
1
Bacterial decolorization of textile dyes is an extracellular process requiring a multicomponent electron transfer pathway.
Microb Biotechnol. 2008 Jan;1(1):40-52. doi: 10.1111/j.1751-7915.2007.00005.x.
2
Enhancing survival of Escherichia coli by expression of azoreductase AZR possessing quinone reductase activity.
Appl Microbiol Biotechnol. 2008 Sep;80(3):409-16. doi: 10.1007/s00253-008-1555-6. Epub 2008 Jun 12.
3
Lateral gene transfer in phylogeny of azoreductase enzyme.
Comput Biol Chem. 2008 Jun;32(3):191-7. doi: 10.1016/j.compbiolchem.2008.03.003. Epub 2008 Mar 26.
4
Expansion of substrate specificity and catalytic mechanism of azoreductase by X-ray crystallography and site-directed mutagenesis.
J Biol Chem. 2008 May 16;283(20):13889-96. doi: 10.1074/jbc.M710070200. Epub 2008 Mar 12.
5
Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis.
Mol Microbiol. 2008 Mar;67(5):1108-24. doi: 10.1111/j.1365-2958.2008.06110.x. Epub 2008 Jan 16.
6
Flavin-dependent quinone reductases.
Cell Mol Life Sci. 2008 Jan;65(1):141-60. doi: 10.1007/s00018-007-7300-y.
7
Anaerobic metabolism of 1-amino-2-naphthol-based azo dyes (Sudan dyes) by human intestinal microflora.
Appl Environ Microbiol. 2007 Dec;73(23):7759-62. doi: 10.1128/AEM.01410-07. Epub 2007 Oct 12.
8
The NADPH quinone reductase MdaB confers oxidative stress resistance to Helicobacter hepaticus.
Microb Pathog. 2008 Feb;44(2):169-74. doi: 10.1016/j.micpath.2006.08.005. Epub 2007 Sep 6.
9
Molecular cloning, characterisation and ligand-bound structure of an azoreductase from Pseudomonas aeruginosa.
J Mol Biol. 2007 Nov 9;373(5):1213-28. doi: 10.1016/j.jmb.2007.08.048. Epub 2007 Aug 25.
10
Azoreductase from Rhodobacter sphaeroides AS1.1737 is a flavodoxin that also functions as nitroreductase and flavin mononucleotide reductase.
Appl Microbiol Biotechnol. 2007 Oct;76(6):1271-9. doi: 10.1007/s00253-007-1087-5. Epub 2007 Sep 11.
Zotero 插件